Digitized landmarks (1–13) for thin plate spline analysis of body morphology and area measured for skin reflectance. Digitized landmarks (1–13) for thin plate spline analysis of body morphology and area measured for skin reflectance. Skin reflectance was quantified in the shaded region behind the pectoral fin, below the lateral line (dashed line), and before the insertion of the dorsal fin as the average white pixel intensity. Image modified from Nichols et al. (2008)‏ Benjamin C. Hecht et al. G3 2015;5:873-889 ©2015 by Genetics Society of America