Protein expression of EAAT2 paralogs.

Slides:

Advertisements

Similar presentations

Presented by Juan Mo. Study Questions Why zebrafish transgenetic lines are very important for studying photoreceptor degeneration? The synapses in inner.

Advertisements

The effects of illumination of VNUT expression in zebrafish retina Kartik Sreepada 1, Xiangping Chu 1, Salvatore L Stella Jr. 2 1 Department of Basic Medical.

From: A Frameshift Mutation in RPGR Exon ORF15 Causes Photoreceptor Degeneration and Inner Retina Remodeling in a Model of X-Linked Retinitis Pigmentosa.

Figure S1 a b c d DAPI Lgr β-catenin Merge

TMZ and Ara-C treatment of slice co-cultures.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

NPM1 drives poly-GR into the nucleolus of primary neurons.

A Mutation in ZNF513, a Putative Regulator of Photoreceptor Development, Causes Autosomal-Recessive Retinitis Pigmentosa Lin Li, Naoki Nakaya, Venkata.

Dendritic spine density at P30 is elevated on isolated PirB−/− neurons in layer 2/3 after sparse excision of PirB at E15.5. Dendritic spine density at.

Density of dendritic spines on L2/3 pyramidal neurons is greater in visual cortex of germline PirB−/− mice than in PirB+/+ mice at P30. Density of dendritic.

Clone patterns and frequencies produced by lines 168 and 688A.

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

A Mutation in ZNF513, a Putative Regulator of Photoreceptor Development, Causes Autosomal-Recessive Retinitis Pigmentosa Lin Li, Naoki Nakaya, Venkata.

Isaac Perea-Gil et al. BTS 2016;1:

Candidates with a PVM localization.

Type 1 NSPCs express nestin, ApoE, and GFAP, while mature granule cells make connections with astrocytes expressing ApoE and GFAP. A–D, Representative.

A1 A2 A3 A4 Figure S1. (A2, C2) hPDLSCs at P2 show low expression of p16 and p21 (red fluorescent) as indicated by double immunostaining. (A3, C3) Cells.

Inhibition of VEGFA or macrophage-specific ablation of Vegfa from TIE2hi/VEGFAhi TMEM macrophages reduces vascular permeability and tumor cell intravasation.

Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs.

Candidates function in the soma to control GSC and niche cap cell number during larval-pupal transition. Candidates function in the soma to control GSC.

Immunohistochemical analysis of individual StanEx enhancer trap line expression in larval brain and VNC. Third larval instar CNS and VNC expression of.

Upper cortical layer-enriched neuronal expression of HA-UPRT.

Absence of a retinal phenotype in CIB2−/− mice

Identification of one type of RGCs with large somas.

HDAC4N increases with Braak stage in CA2 hippocampal pyramidal cells.

Neuronal ATM level is reduced in mouse models of AD

Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Expression of pcdh19 in BAC transgenic line.

Clonally derived neurosphere efficiently contributes to spinal cord regeneration and reconstitutes the whole complement of spinal cord cell types. Clonally.

Volume 25, Issue 4, Pages (May 2013)

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Volume 47, Issue 2, Pages (July 2005)

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Volume 19, Issue 9, Pages (September 2011)

Fig. 6. Meis1 protein is present in CR4. 2-GFP+ and Foxn4+ cells

Two-photon imaging of GCaMP6s in zebrafish larvae.

Insulin promoter confers β-cell–specific GFP expression in pancreas.

Fibroblasts from Rothmund–Thomson syndrome patients have abnormal spindle axis and more micronuclei. Fibroblasts from Rothmund–Thomson syndrome patients.

The conserved region upstream of wt1a is required for its expression in the intermediate mesoderm. The conserved region upstream of wt1a is required for.

Fluorescence confocal micrograph images of human ciliated nasal epithelial cells showing presence (normal) or absence (examples here from different primary.

PTX-induced reporter protein expression in the brain of the IEG reporter line. PTX-induced reporter protein expression in the brain of the IEG reporter.

Adult conditional gene deletion of PGC-1α.

Characterization of NEX-CRE mice and analysis of Itgb1 expression by flow cytometry. a–n , Z/EG reporter mice carrying a CRE-inducible GFP transgene were.

The adult female mouse ARC and VMH generate newborn neurons that respond to estrogens and leptin. The adult female mouse ARC and VMH generate newborn neurons.

Activation of glial cells.

Fig. 4. CR4.2 may be active in amacrine cells but not in ganglion cells.Chick retinas were electroporated with either the control CAG-GFP construct or.

Fig. 2. Acetylation stiffens primary cilia.

RA signaling in early postnatal PFC

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

c-Fos in RFMes GABAergic neurons across groups.

Macaque Satb2-RGCs are synaptically connected to geniculate neurons.

SiRNA directed at Ngn1 inhibited differentiation of inner ear stem cells to β-III tubulin-positive cells. a, Inner ear stem cells treated with siRNA to.

Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs;

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

Expression of Chx10-cre and phenotypes of Chx10-cre induced Smo ablation. Expression of Chx10-cre and phenotypes of Chx10-cre induced Smo ablation. A–G,

Identification of a novel subset of mDA neurons in the VTA that expresses Neurod6, OTX2, CALBINDIN1, ALDH1A1, and Grp. Identification of a novel subset.

Topographic organization of CA3 to CA1 projections revealed through monosynaptic rabies tracing by specifically targeting CA1 pyramidal cells along the.

Proprioceptive sensory neurons and muscle spindles are marked reduced in adult Swl/+ mice. Proprioceptive sensory neurons and muscle spindles are marked.

Effects the genotypes of Rapgef2 and Rapgef6 on RG fiber organization and neuronal migration. Effects the genotypes of Rapgef2 and Rapgef6 on RG fiber.

Coexpression of Crf and Tac2 (A-P -1.5).

Specificity of the WDR81 antibody for the antigen.

Quantification of TUNEL staining and OS length among the rab11a constructs. Quantification of TUNEL staining and OS length among the rab11a constructs.

PC loss in Nhe6-null mouse cerebellum, female and male.

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains.

Coexpression of Crf, Tac2, and Prkcd; and Sst, Tac2, and Prkcd (A-P -0

The predicted new isoform of PCP2 is expressed in retinal ON bipolar cells. The predicted new isoform of PCP2 is expressed in retinal ON bipolar cells.

BDNF protein is expressed in neurons and astrocytes of hippocampus after pilocarpine-induced SE. A, Representative confocal images of hippocampal subfields.

Presentation transcript:

Protein expression of EAAT2 paralogs. Protein expression of EAAT2 paralogs. A–F, Double immunostaining of EAAT2a (green) and glutamine synthetase (magenta) in adult (A) and larval (5 dpf; D) retinal sections confirm expression of EAAT2a in Müller glia cells. Separated channels are shown in B (adult), E (5 dpf; EAAT2a, green channel only), C (adult), and F (5 dpf; glutamine synthetase, magenta channel only). Scale bar in A is 30 µm; also applies to B and C. Scale bar in D is 50 µm; also applies to E and F. G–N, EAAT2b protein is expressed in a dotted manner in the outer plexiform layer (OPL) in all cone pedicles, but it is not expressed in rods. EAAT2b antibody staining (magenta) on adult retinal sections stained with Zpr-1 (red-green double cones, G) and on retinal sections of zebrafish expressing GFP in blue cones (H), UV cones (I), and rods (J) confirms that EAAT2b is cone specific and is spared from rod spherules. K–M show zoom-ins of the cone pedicles expressing EAAT2b (magenta) in red-green double cones (K), blue cones (L), and UV cones (M). N shows larval (5 dpf) expression of EAAT2b in magenta together with a nuclear counterstain (DAPI, blue). Scale bars in G–J are 7 µm. Scale bars in K–M are 2 µm. Scale bar in N is 30 µm. Stephanie Niklaus et al. eNeuro 2017;4:ENEURO.0339-16.2017 ©2017 by Society for Neuroscience