In Vivo Fluorometric Assessment of Cyclosporine on Mitochondrial Function During Myocardial Ischemia and Reperfusion Muneaki Matsubara, MD, Mahsa Ranji,

Slides:

Advertisements

Similar presentations

Morphologic Spectrum of Ventriculoarterial Connection in Hearts With Double Inlet Left Ventricle: Implications for Surgical Procedures Hideki Uemura, MD,

Advertisements

Predicted Risk of Mortality, Transfusion, and Postoperative Outcomes in Isolated Primary Valve Operations W. Brent Keeling, MD, Jose Binongo, PhD, Eric.

An ovine model of postinfarction dilated cardiomyopathy

Michael A. Long, MMed The Annals of Thoracic Surgery

Allogeneic Mesenchymal Precursor Cell Therapy to Limit Remodeling After Myocardial Infarction: The Effect of Cell Dosage Hirotsugu Hamamoto, MD, Joseph.

Bing-Yen Wang, MD, Hsiao-Huang Chang, MD, PhD, I

Mark K. Ferguson, MD, Jeanne Farnan, MD, MHPE, Josh A

Liam P. Ryan, MD, Kanji Matsuzaki, MD, PhD, Mio Noma, MD, Benjamin M

Is the Myofibrillarlytic Myocyte a Forme Fruste Apoptotic Myocyte?

Changfa Guo, MD, Husnain Kh

Walter R. T. Witschey, PhD, Francisco Contijoch, MS, Jeremy R

Cytokines Link Toll-Like Receptor 4 Signaling to Cardiac Dysfunction After Global Myocardial Ischemia John Cha, MD, Zhiping Wang, MD, Lihua Ao, BS, Ning.

Application of the Human Factors Analysis and Classification System Methodology to the Cardiovascular Surgery Operating Room Andrew W. ElBardissi, BS,

Osteopontin Combined With CD44v6, a Novel Prognostic Biomarker in Non-Small Cell Lung Cancer Undergoing Curative Resection Bing-sheng Sun, MD, PhD, Yue.

Value of Magnetic Resonance Imaging, Angiography, and Fractional Flow Reserve to Evaluate the Left Main Coronary Artery After Direct Surgical Angioplasty

Severity of Preoperative Aortic Regurgitation Does Not Impact Valve Durability of Aortic Valve Repair Following the David V Valve Sparing Aortic Root.

Burdens Without Blessings: Peer Reviewers Get No Respect

Anomalous Great Cardiac Vein Draining Into the Superior Vena Cava

Expression and Function of Endothelial Nitric Oxide Synthase Messenger RNA and Protein Are Higher in Internal Mammary Than in Radial Arteries Guo-Wei.

Tadeusz Wellisz, MD, Jonathan K

Mammalian Fetal Cardiac Regeneration After Myocardial Infarction Is Associated With Differential Gene Expression Compared With the Adult Carlos Zgheib,

Sarah M. Brant, MD, Michael L. Cobert, PhD, LaShondra M

Proteasome Inhibition Attenuates Infarct Size and Preserves Cardiac Function in a Murine Model of Myocardial Ischemia-Reperfusion Injury William E. Stansfield,

Susanne Lison, MD, Michael Spannagl, MD, PhD, Wulf Dietrich, MD, PhD

Pleural Metastasis of Osteosarcoma

Hirotsugu Hamamoto, MD, Bradley G. Leshnower, MD, Landi M

Origins of the Cardiothoracic Surgery Network (CTSNet)

Who Do You Say You Are? The Annals of Thoracic Surgery

Potent adenylate cyclase agonist forskolin restores myoprotective effects of ischemic preconditioning in rat hearts after myocardial infarction Shigetoshi.

Todd C. Crawford, MD, J. Trent Magruder, MD, Joshua C

The Relationship Between Hospital Surgical Case Volumes and Mortality Rates in Pediatric Cardiac Surgery: A National Sample, 1988–2005 Karl F. Welke,

Use of Amiodarone After Major Lung Resection

Surgical Dilemmas: Diagnosis and Treatment of Atrial Plasmacytoma

Periarteritis in Lung From a Continuous-Flow Right Ventricular Assist Device: Role of the Local Renin-Angiotensin System Chiyo Ootaki, MD, Michifumi.

Temporal Changes in Infarct Material Properties: An In Vivo Assessment Using Magnetic Resonance Imaging and Finite Element Simulations Jeremy R. McGarvey,

Dose-dependent neuroprotection of delta-opioid peptide [D-Ala2, D-Leu5] enkephalin on spinal cord ischemia-reperfusion injury by regional perfusion into.

Injectable Microsphere Gel Progressively Improves Global Ventricular Function, Regional Contractile Strain, and Mitral Regurgitation After Myocardial.

Aprotinin Exerts Differential and Dose-Dependent Effects on Myocardial Contractility, Oxidative Stress, and Cytokine Release After Ischemia-Reperfusion

Modification of Infarct Material Properties Limits Adverse Ventricular Remodeling Masato Morita, MD, Chad E. Eckert, PhD, Kanji Matsuzaki, MD, PhD, Mio.

Anomalous Origin of Left Pulmonary Artery From Left Subclavian Artery

Hemodynamic Assessment After Complete Repair of Pulmonary Atresia With Major Aortopulmonary Collaterals Richard D. Mainwaring, MD, V. Mohan Reddy, MD,

Embolized Methyl Methacrylate to the Right Atrium After Kyphoplasty

Editorial Board, January 2010

Sarah C. Huen, MD, Chirag R. Parikh, MD, PhD

Elimination of Ischemic Mitral Regurgitation Does Not Alter Long-Term Left Ventricular Remodeling in the Ovine Model Kanji Matsuzaki, MD, PhD, Masato.

Shinya Kanemoto, MD, Muneaki Matsubara, MD, Mio Noma, MD, Bradley G

Completely Thrombosed Tricuspid Pouch Mimicking a Cardiac Tumor

Surgical Treatment of Aortic Prosthetic Valve Endocarditis: A 20-Year Single-Center Experience Sossio Perrotta, MD, Anders Jeppsson, MD, PhD, Victoria.

An Unusual Cause of Hemoptysis in a Young Woman

Current Assessment of Mortality Rates in Congenital Cardiac Surgery

The Annals of Thoracic Surgery

Brian M. Cardis, MD, Derek A. Fyfe, MD, PhD, William T. Mahle, MD

Anirudh Vinnakota, MS, Robert Stewart, MD, Gosta Pettersson, MD, PhD

Transmyocardial laser revascularization: experimental studies in healthy porcine myocardium Georg Lutter, MD, Juergen Martin, MD, Noriyuki Takahashi,

Adenosine-enhanced ischemic preconditioning provides myocardial protection equal to that of cold blood cardioplegia James D McCully, PhD, Masahisa Uematsu,

Survival After Isolated Coronary Artery Bypass Grafting in Patients With Severe Left Ventricular Dysfunction Waleed A. Ahmed, MD, Phillip J. Tully, BHSc(Hons),

Age- and Gender-Related Differences in Mitochondrial Oxygen Consumption and Calcium With Cardioplegia and Diazoxide James D. McCully, PhD, Anthony J.

Invited Commentary The Annals of Thoracic Surgery

Robert Lee Replogle, September 30, 1931–May 9, 2016

The American Board of Thoracic Surgery: Update

“Transitions” for Cardiothoracic Surgeons Now Listed on the Web:

Increased Rehospitalization Rate After Coronary Bypass Operation for Acute Coronary Syndrome: A Prospective Study in 200 Patients Staffan Bjessmo, MD,

Mark I. Block, MD The Annals of Thoracic Surgery

Late Consequences of a Much Earlier Decision

Effects of in vivo myocardial ischemia and reperfusion on interstitial nitric oxide metabolites Randy M Stevens, MD, M.Salik Jahania, MD, Jennifer E.

The Impact of New Technology on Cardiothoracic Surgical Practice

Self-Management of Oral Anticoagulation Therapy Improves Long-Term Survival in Patients With Mechanical Heart Valve Replacement Heinrich Koertke, MD,

Erik Madssen, RF, Petter Haere, MD, Rune Wiseth, MD, PhD

Surgical Reconstruction of Peripheral Pulmonary Arteries: Strategies, Outcomes, and New Classification Abdulaziz Al-Khaldi, MD, MS, Omar Tamimi, MD

J. Alberto Lopez, MD The Annals of Thoracic Surgery

Presentation transcript:

In Vivo Fluorometric Assessment of Cyclosporine on Mitochondrial Function During Myocardial Ischemia and Reperfusion Muneaki Matsubara, MD, Mahsa Ranji, PhD, Bradley G. Leshnower, MD, Mio Noma, MD, Sarah J. Ratcliffe, PhD, Britton Chance, PhD, Robert C. Gorman, MD, Joseph H. Gorman, MD The Annals of Thoracic Surgery Volume 89, Issue 5, Pages 1532-1537 (May 2010) DOI: 10.1016/j.athoracsur.2010.01.065 Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Comparison of area at risk (AR) and infarct sizes (IS) among the untreated (UnT), cyclosporine A (CsA) preinfarction (CsAp), and CsA reperfusion (CsAr) groups. Mean values are shown with the standard error of the mean (error bars). The area at risk is expressed as a percentage of the left ventricle, and the infarct size is expressed as a percentage of the area at risk. The area at risk was not significantly different between groups. Infarct size was significantly greater in the untreated group than in either of the CsA groups (*p < 0.01). Infarct size was the same in both CsA groups. The Annals of Thoracic Surgery 2010 89, 1532-1537DOI: (10.1016/j.athoracsur.2010.01.065) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Comparison of mean redox ratio (RR) between the untreated (UnT; black circle), cyclosporine A (CsA) preinfarction (CsAp; white circle) and CsA reperfusion (CsAr; black triangle) groups throughout the time course of the experiment. There were no significant differences among the groups during the baseline or infusion phases. During ischemia, the CsAp curve is significantly different from the UnT and CsAr groups. During reperfusion, all three curves were significantly different. The average rate of increase of the redox ratio during the first 70 minutes of reperfusion, as assessed by a best-fit line through all the data points, was significantly greater in the UnT and CsAr groups than it was in the CsAp group (0.005 min−1 vs 0.003 min−1; p < 0.001). After 90 minutes of reperfusion, the CsAr curve began to deviate from the UnT curve. Error bars represent standard error of the mean. The Annals of Thoracic Surgery 2010 89, 1532-1537DOI: (10.1016/j.athoracsur.2010.01.065) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Representative transmission electron photomicrographs of rabbit myocardium images (original magnification ×30,000) compare the differences among the mitochondria of the (A) normal, (B) untreated, (C) cyclosporine A (CsA) preinfarction, and (D) CsA reperfusion groups. The mitochondria of the normal myocardium are tightly packed and possess an intact mitochondrial membrane and well-organized cristae, unlike the untreated group, that is characterized by swollen, loosely packed, vacuolated, and completely disrupted mitochondria. Although the CsA preinfarction and reperfusion groups demonstrate mitochondria with dilated cristae, their general morphology is much closer to normal than was the untreated group. The Annals of Thoracic Surgery 2010 89, 1532-1537DOI: (10.1016/j.athoracsur.2010.01.065) Copyright © 2010 The Society of Thoracic Surgeons Terms and Conditions