Dermal cells from human neonatal foreskin can be distinguished into fibroblasts and pericytes based on differential VLA-1 expression. Dermal cells from.

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Volume 9, Issue 5, Pages (November 2017)

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Dermal cells from human neonatal foreskin can be distinguished into fibroblasts and pericytes based on differential VLA-1 expression. Dermal cells from human neonatal foreskin can be distinguished into fibroblasts and pericytes based on differential VLA-1 expression. (A, B) Immunostaining of human neonatal skin with (A) αSMA (green) and FAPα (red) or (B) VLA-1 (green) and FAPα (red) showing mutually exclusive staining of pericytes with αSMA and VLA-1 in capillaries and fibroblasts with FAPα. VLA-1 is undetectable by in situ immunofluorescence staining in fibroblasts because of its significantly lower levels of expression in these cells. Nuclei stained with DAPI (white); c, capillary; e, epidermis; white arrows indicate FAPα-positive dermal fibroblasts. (C) Flow cytometric analysis of VLA-1 (x-axis) versus FAPα (y-axis) expression in freshly isolated CD45−dermal cells from neonatal human skin, revealing that VLA-1dim cells are exclusively FAPα positive, whereas VLA-1bri cells previously shown by us to be pericytes are negative for FAPα. Lizhe Zhuang et al. LSA 2018;1:e201700009 © 2018 Zhuang et al.