TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics

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TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics. TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics. (A) STx2B transport assay in cells treated with vehicle DMSO, TOR, or OSP for 24 h. Scale bars, 25 μm. (B) STx2B levels at 60 min from (A). Levels in DMSO-treated cells are normalized to 100. N > 25 cells per condition. *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for the comparison between DMSO and other groups. (C) Lysosensor staining in cells treated with indicated compounds for 30 min. Scale bar, 25 μm. (D) Quantification of mean Lysosensor signal per cell from (C). Signal in DMSO-treated cells is normalized to 100. N = 25 cells per condition. *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for comparison between DMSO and other groups. (E) Immunofluorescence to detect EEA1 and Vps26 in cells exposed to DMSO or indicated compounds for 24 h. Scale bar, 25 μm. (F) Quantification of data from (E). N ≥ 15 cells per condition. *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for comparison between DMSO and other groups. Andrey S Selyunin et al. LSA 2019;2:e201900439 © 2019 Selyunin et al.