Fig. 1. Scratching monolayers stimulates cAMP synthesis in MDCK cells.

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Volume 48, Issue 6, Pages (June 2011)

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Fig. 1. Scratching monolayers stimulates cAMP synthesis in MDCK cells. Scratching monolayers stimulates cAMP synthesis in MDCK cells. Confluent monolayers of MDCK cells were maintained in 1.8 mM Ca2+ Ringer's solution containing 100 µM IBMX, a phosphodiesterase inhibitor. Cells were then wounded by scratching monolayers with a 27G needle 20 times, or treated with 100 µM ATP or 100 µM forskolin. The amount of cAMP was quantified by immunoassay and normalized to the amount of total protein. The number of experiments is indicated in parentheses. *P=0.0006. Tatsuru Togo Biology Open 2017;6:1814-1819 © 2017. Published by The Company of Biologists Ltd