Histological analysis of intravitreal injection of metabolically inactive B. cereus, S. aureus, and E. faecalis. Histological analysis of intravitreal.

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Histological analysis of intravitreal injection of metabolically inactive B. cereus, S. aureus, and E. faecalis. Histological analysis of intravitreal injection of metabolically inactive B. cereus, S. aureus, and E. faecalis. Metabolically inactive B. cereus (106 CFU), S. aureus (106 CFU), and E. faecalis (108 CFU) were injected into the vitreous. Injection inocula were chosen based upon the number of viable organisms present when early signs of infection were observed in the natural infection (20). Representative eyes were harvested at 72 h postinfection. Vitreous was sham injected (A) or injected with B. cereus (B), E. faecalis (C), or S. aureus (D). Injection of metabolically inactive organisms (B to D) caused extensive influx of inflammatory cells and fibrin accumulation in the posterior chamber by 72 h postinfection. Retinal layers remained intact. All sections were stained with hematoxylin-eosin. Magnification, ×270. Abbreviations: CH, choriocapillaris; RET, retina; VIT, vitreous; IC, inflammatory cells. Michelle C. Callegan et al. Clin. Microbiol. Rev. 2002; doi:10.1128/CMR.15.1.111-124.2002