Connecting Replication and Recombination Susan T. Lovett  Molecular Cell  Volume 11, Issue 3, Pages 554-556 (March 2003) DOI: 10.1016/S1097-2765(03)00110-2 Copyright © 2003 Cell Press Terms and Conditions

Figure 1 Possible Scheme for PriA-Dependent Control of Replication Fork Establishment A broken arm of the replication fork is processed by RecBCD. RecA (purple ovals) is loaded and a D loop recombination intermediate is formed between the broken arm and the sister chromosome. If PriA does not bind, DNA synthesis from the invading 3′ end would merely extend the D loop without forming a processive replication fork. If PriA (turquoise hexagon) does bind to the invading 3′ end, DNA synthesis is inhibited while PriA (with PriBC DnaCT) loads DnaB helicase (gold ring) to the displaced strand of the D loop. DnaB recruits DnaG primase (green circle), and this relieves PriA inhibition to allow both lagging- and leading-strand DNA synthesis to begin via DNA polymerase III holoenzyme (pink triangles). In contrast, a broken chromosome may engage both broken arms into the D loop, thereby potentially blocking DnaB loading; repair may be accomplished with a small amount of repair synthesis, without replication fork establishment. Molecular Cell 2003 11, 554-556DOI: (10.1016/S1097-2765(03)00110-2) Copyright © 2003 Cell Press Terms and Conditions