Fig. 1 In vivo and in organello analysis of the CL dependence of the Tim23-Tim50 interaction. In vivo and in organello analysis of the CL dependence of.

Slides:

Advertisements

Similar presentations

Fig. 5 Correlation of RNA expression and protein abundance.

Advertisements

Fig. 2 The SOS response is required for persistence to ofloxacin but not prior treatment. The SOS response is required for persistence to ofloxacin but.

Fig. 2 Transport properties of a BP transistor at low temperature.

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 3 Vibrational spectra of human bones from the Copper Age (Scoglietto cave, Italy). Vibrational spectra of human bones from the Copper Age (Scoglietto.

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Map of water stress and shale plays.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 3 Electron PSD in various regions.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

Fig. 1 Importance of boronic acids, commonly used synthetic methods for the ─B(OH)2 introduction, and our proposed building block–centered approach. Importance.

HT synthesis of boronic acids using the building block approach

Fig. 6 Comparison of properties of water models.

Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.

Fig. 2 Reference-fixing experiment, results.

Fig. 2 The glucose binding and charge-switch study.

Fig. 3 Glucose- and structure-dependent insulin release.

Fig. 2 TRXSS data. TRXSS data. TRXSS data covering delay times from 10 ns to 10 ms for wild-type DmCry (A), for the DmCry(H378A) mutant (B), and for XlPho.

Fig. 2 Stratigraphic profile of the Area 15 excavation block showing the diagnostic cultural materials and components alongside the stratigraphic sequence.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

FTIR-ATR spectra of Neolithic faunal bones (Mora Cavorso cave, Italy)

Fig. 3 Gate voltage dependence of the areal iDMI and PMA.

Fig. 1 Concept of the livestock transition in China between 1980 and Concept of the livestock transition in China between 1980 and The left-

Fig. 5 GP38 localization to the viral envelope and plasma membrane.

Fig. 1 Distribution of total and fake news shares.

Fig. 5 Analysis of VLPs formed from deletion CP mutants.

Fig. 2 2D QWs of different propagation lengths.

Fig. 4 Organophosphorus compounds detected in the room temperature residues irradiated ices by multidimensional gas chromatography. Organophosphorus compounds.

Fig. 5 Molecular dynamics simulations of Rac1.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 4 The mechanical performances of thermally stable click-ionogels.

Fig. 1 Cross-sectional images of He-implanted V/Cu/V samples.

Fig. 4 Evolution of fraction of sickled RBCs under hypoxia.

Fig. 2 Magnetic properties of FGT/Pt bilayer.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 5 The different fractions of the rates of energy flow in the oscillating thermal circuit for the experiment with L = 58.5 H shown in Fig. 4A. The.

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 1 Size fractions of MPPs in different fertilizers.

Fig. 2 Sampling. Sampling. (A) Extant stratigraphic section. Zenithal (B) and frontal (C) views of the flowstone capping the excavated deposit. The rectangle.

Fig. 2 Top 10 countries, ecoregions, conservation hotspots, and KBAs with the largest area of restoration hotspots. Top 10 countries, ecoregions, conservation.

Fig. 4 Reactive MD simulation showing the origins of chemical and physical effects on friction. Reactive MD simulation showing the origins of chemical.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 5 The VWF A2 domain exists predominantly in a reduced dithiol and glutathionylated form in healthy donors. The VWF A2 domain exists predominantly.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 2 Average reduction in grain micronutrients, iron (Fe), and zinc (Zn) concentration at elevated relative to ambient [CO2] for 18 cultivated rice lines.

Fig. 6 MSC encapsulation in vitro within PdBT cross-linked gels.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 2 Mean field results. Mean field results. (A) Solutions P(x) to Eq. 4 for a range of T and wc = (B) Modulus ∣pk∣ of order parameters versus.

Fig. 3 Comparisons of NDVI trends over the globally vegetated areas from 1982 to Comparisons of NDVI trends over the globally vegetated areas from.

Fig. 2 Particles detected in snow samples collected at different locations from Europe to the Arctic. Particles detected in snow samples collected at different.

Fig. 1 Schematic depiction of a paradigm for rapid and guided discovery of materials through iterative combination of ML with HiTp experimentation. Schematic.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 5 The complex of antibody and dye can afford to target imaging in the NIR-II window. The complex of antibody and dye can afford to target imaging.

Fig. 4 Behavior of resistance peak near density nm = 5.

Fig. 3 TBK1 phosphorylates RAB7AS72 but not the equivalent residue in other RABs in vitro. TBK1 phosphorylates RAB7AS72 but not the equivalent residue.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 4 Effects of individual picosecond and microsecond pulses.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 6 Schematic illustration of the decreased conformational stability in the N92I mutant and its activation mechanism. Schematic illustration of the.

Fig. 5 CD19-tPSMA(N9del) CAR T cell numbers in mouse and human.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

Fig. 1 In vivo and in organello analysis of the CL dependence of the Tim23-Tim50 interaction. In vivo and in organello analysis of the CL dependence of the Tim23-Tim50 interaction. (A) Coimmunoprecipitation analysis of the Tim23-Tim50 interaction. Digitonin lysates of mitochondria from yeast strains containing full-length Tim50 (left) or Tim50IMS (right) were immunoprecipitated with affinity-purified antibodies to Tim23 (αTim23) or Tim50 (αTim50) or preimmune immunoglobulins (PI). Unbound (Sup.) and bound (Pellets) fractions were analyzed by SDS-PAGE and immunodecoration with antibodies against Tim50, Tim23, or Tim17, as indicated. Total and Sup. lanes indicate 20% of starting material; all other lanes represent undiluted samples. (B) Synthetic lethality of tim23yl and Tim50IMS. Yeast cells containing a chromosomal deletion of TIM23 complemented with a TIM23-containing URA3 plasmid were generated in a Tim50 or Tim50IMS background (delineated by dotted boxes). Cells were transformed with centromeric plasmids carrying either Tim23 or tim23yl, as indicated, and subjected to URA3 plasmid loss by plating on glucose-containing medium with 5-fluoroorotic acid (5-FOA). Cells were incubated at 30°C (upper plate) or 24°C (lower plate). (C) Genetic interaction of Δcrd1 and tim23yl. Tenfold serial dilutions were prepared from yeast cells containing WT Tim23 or the tim23yl mutant in a background of normal CL synthesis or CL synthase knockouts (Δcrd1). Cells were spotted onto YPD or YPLac medium as shown and grown at the indicated temperatures. Ketan Malhotra et al. Sci Adv 2017;3:e1700532 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).