Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

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Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. (A) The results of flow cytometric analysis of STS-treated CHO cells incubated with HIS6X–GFP–D4 protein. Cells were incubated with STS (50 nM) for 24 h. For rescue experiments, STS-treated CHO cells were incubated with lipids for 2 h. (B) Confocal images of STS-treated CHO cells expressing mCherry–D4H and GFP–Lact-C2. Scale bars: 10 µm. (C) Quantification of the data shown in Fig. 6B. PM, plasma membrane. A total of 100 cells from three independent experiments were analyzed. The data show the mean±s.e.m.; *P<0.05; **P<0.01. Masashi Maekawa, and Gregory D. Fairn J Cell Sci 2015;128:1422-1433 © 2015. Published by The Company of Biologists Ltd