Motoki Yano, MDa, Carlos H. R. Boasquevisque, MDa, Ronald K

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Successful in vivo and ex vivo transfection of pulmonary artery segments in lung isografts Motoki Yano, MDa, Carlos H.R. Boasquevisque, MDa, Ronald K. Scheule, PhDc, Mitchell D. Botney, MDb, Joel D. Cooper, MDa, G.Alexander Patterson, MDa The Journal of Thoracic and Cardiovascular Surgery Volume 114, Issue 5, Pages 793-802 (November 1997) DOI: 10.1016/S0022-5223(97)70083-7 Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Recombinant gene expression with Bluo-Gal staining on the endothelial surface. In vivo transfection (group II) (A) and ex vivo transfection (group IV) (B) are seen with blue spots through the microscope (original magnification ×100). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Recombinant gene expression with Bluo-Gal staining on the endothelial surface. In vivo transfection (group II) (A) and ex vivo transfection (group IV) (B) are seen with blue spots through the microscope (original magnification ×100). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Recombinant gene expression with Bluo-Gal staining on the pathologic specimens of a pulmonary artery segment. In vivo transfection (group II) (A) and ex vivo transfection (group IV) (B) are seen with blue spots on the endothelial cells and smooth muscle cells stained with nuclear fast red stain (original magnification ×400). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Recombinant gene expression with Bluo-Gal staining on the pathologic specimens of a pulmonary artery segment. In vivo transfection (group II) (A) and ex vivo transfection (group IV) (B) are seen with blue spots on the endothelial cells and smooth muscle cells stained with nuclear fast red stain (original magnification ×400). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 CAT activity in groups V and VI. In the lipid group (VI), strong and consistent expression of CAT is apparent. di-AC, Diaceylated; mono-AC, monoaceylated; non-AC, nonaceylated. The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 In situ hybridization in group VI. CAT gene was detected with concentration of positive signals around endothelial cells using both bright-field microscopy (A) and dark-field microscopy with reflected polarized light (B) (original magnifications ×200). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 In situ hybridization in group VI. CAT gene was detected with concentration of positive signals around endothelial cells using both bright-field microscopy (A) and dark-field microscopy with reflected polarized light (B) (original magnifications ×200). The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 5 Arterial oxygen tension in groups III to VI. There are no differences between corresponding groups. NS, Not significant. The Journal of Thoracic and Cardiovascular Surgery 1997 114, 793-802DOI: (10.1016/S0022-5223(97)70083-7) Copyright © 1997 Mosby, Inc. Terms and Conditions