Enzyme activity in the hypothalamus and hindbrain of rainbow trout after GHRL and/or ceramide treatment. Enzyme activity in the hypothalamus and hindbrain of rainbow trout after GHRL and/or ceramide treatment. Activity of CPT-1 (A,D), FAS (B,E), ACLY (C,F) and HOAD (G) in the hypothalamus (A–C) and hindbrain (D–G) of rainbow trout 6 h after ICV administration of 1 µl 100 g−1 body mass of DMSO-saline (1:3) alone (control, C), or containing 200 ng of rainbow trout GHRL (G), 2.5 μg of C6:0 ceramide (CE) or GHRL+ceramide (G+CE). Each value is the mean+s.e.m. of n=9 fish per treatment. Different letters indicate significant differences (P<0.05) between groups. Cristina Velasco et al. J Exp Biol 2017;220:2563-2576 © 2017. Published by The Company of Biologists Ltd