What I expect to see in Skyline

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Presentation transcript:

What I expect to see in Skyline Precursor 175.1189 at MS low CE What I expect to see in Skyline Product 116.0706 at MS high CE Precursor 175.1189 at MS high CE What I see in Skyline Product 116.0706 at MS low CE Precursor Products

at MS high CE at MS high CE at MS low CE at MS low CE Precursor 175.1189 Precursor 175.1189 at MS high CE at MS high CE Product 116.0706 at MS low CE Product 116.0706 at MS low CE

at MS low CE at MS high CE at MS high CE at MS low CE Precursor 175.1189 Precursor 175.1189 at MS low CE at MS high CE Product 116.0706 at MS high CE Product 116.0706 at MS low CE

(looking at mass spectra) What I see in Skyline (looking at mass spectra) Skyline Set-up Precursor 175.1189 at MS high CE Product 116.0706 at MS low CE