Effects of pregabalin on the activity of glutamate transporter type 3

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Effects of pregabalin on the activity of glutamate transporter type 3 J.H. Ryu, P.B. Lee, J.H. Kim, S.H. Do, C.S. Kim British Journal of Anaesthesia Volume 109, Issue 2, Pages 234-239 (August 2012) DOI: 10.1093/bja/aes120 Copyright © 2012 The Author(s) Terms and Conditions

Fig 1 Concentration–response curve of EAAT3 to pregabalin. Oocytes injected with EAAT3 mRNA were exposed to pregabalin (0.06–60 μM) for 72 h and exposed to 30 μM glutamate. Data are mean (sd) (n=20–25 in each group). EAAT3, excitatory amino acid transporter type 3. British Journal of Anaesthesia 2012 109, 234-239DOI: (10.1093/bja/aes120) Copyright © 2012 The Author(s) Terms and Conditions

Fig 2 Concentration–response curve of EAAT3 to 30, 100, and 300 μM glutamate in the presence or absence of 0.6 μM pregabalin for 72 h. Inset graph is a Lineweaver–Burk plot using the mean value of the each group with abscissa for the reciprocal of l-glutamate concentration and ordinate for the reciprocal of response (μC). R2=0.96 for the control and pregabalin groups. Data are mean (sd) (n=25 in each group). *P<0.05 compared with the corresponding control. British Journal of Anaesthesia 2012 109, 234-239DOI: (10.1093/bja/aes120) Copyright © 2012 The Author(s) Terms and Conditions

Fig 3 Effects of PKC activation with PMA (100 nM) for 10 min on EAAT3 activity in oocytes in the presence or absence of 0.6 μM pregabalin for 72 h. Data are mean (sd) (n=17–25 in each group). *P<0.05 compared with control. British Journal of Anaesthesia 2012 109, 234-239DOI: (10.1093/bja/aes120) Copyright © 2012 The Author(s) Terms and Conditions

Fig 4 Effects of PKC inhibition by staurosporine (2 µM) or chelerythrine (100 µM) for 1 h on EAAT3 activity in oocytes in the presence or absence of 0.6 μM pregabalin for 72 h. Data are mean (sd) (n=10–19 in each group). *P<0.05 compared with control. †P<0.05 compared with pregabalin. British Journal of Anaesthesia 2012 109, 234-239DOI: (10.1093/bja/aes120) Copyright © 2012 The Author(s) Terms and Conditions

Fig 5 Effects of PI3K inhibition with wortmannin (10 µM) for 1 h on EAAT3 activity in oocytes in the presence or absence of pregabalin (0.6 μM) for 72 h. Data are mean (sd) (n=15–19 in each group). *P<0.05 compared with control. †P<0.05 compared with pregabalin. British Journal of Anaesthesia 2012 109, 234-239DOI: (10.1093/bja/aes120) Copyright © 2012 The Author(s) Terms and Conditions