Ion channels expression and insulin secretion in the pancreatic islets of male offspring. Ion channels expression and insulin secretion in the pancreatic.

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Ion channels expression and insulin secretion in the pancreatic islets of male offspring. Ion channels expression and insulin secretion in the pancreatic islets of male offspring. Representative mRNA levels of Abcc8 (A), Cav1.2 (B), and Cav2.3 (C) in islets of 20-week-old male offspring (nCtrl-F1_NCD = 7, nINS-F1_NCD = 6, nGDM-F1_NCD = 8, nCtrl-F1_HFD = 5, nINS-F1_HFD = 6, nGDM-F1_HFD = 6). D–G: Representative protein levels of Abcc8, Cav1.2, and Cav2.3 in islets of 20-week-old male offspring (n = 4 mice per group). H: Tolbutamide (200 μmol/L) stimulated insulin secretion. I: Diazoxide (250 μmol/L) inhibited insulin secretion. J: Bay K8644 (2 μmol/L) stimulated insulin secretion. K: Nifedipine (10 μmol/L) inhibited insulin secretion. Isolated 20-week-old islets, n = 5 mice per group. Data are expressed as mean ± SEM. *P < 0.05 vs. Ctrl-F1, **P < 0.01 vs. Ctrl-F1; #P < 0.05 vs. INS-F1; §P < 0.05 vs. Ctrl-F1_NCD, §§P < 0.01 vs. Ctrl-F1_NCD (ANOVA). Hong Zhu et al. Diabetes 2019;68:696-708 ©2019 by American Diabetes Association