High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

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High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. (A) Images of β1-integrin-deficient GE11 cells expressing indicated β3 integrin affinity mutants stained for paxillin (green), F-actin (red) and the nucleus (blue). Scale bar: 10 μm. (B) Western blot analysis of RhoA activity assay on lysates of GE11 cells expressing indicated constructs. Quantification shows relative RhoA activation ± s.d. compared with GEβ1 cells from two experiments. (C) Western blot analysis of assembled fibronectin-biotin and vimentin (loading control) in DOC-insoluble lysates of GE11 cells expressing indicated constructs. Stephan Huveneers et al. J Cell Sci 2008;121:2452-2462 © The Company of Biologists Limited 2008