Comparative genomic expressive hybridisation detection and quantification. Comparative genomic expressive hybridisation detection and quantification. (A)

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Comparative genomic expressive hybridisation detection and quantification. Comparative genomic expressive hybridisation detection and quantification. (A) Amplified cDNA hybridised to chromosomes without reference DNA. The left hand image shows hybridisation of cDNA obtained from the Colo320 cell line to chromosome 8q24 (arrow), which contains the locus for the c-myc oncogene, and denotes overexpression of that locus. The right hand image represents hybridisation of cDNA obtained from the T47D cell line to chromosome 3. (B) An example of expressive genomic hybridisation (EGH) using amplified cDNA from the T47D cell line labelled with digoxigenin (red) co-hybridized with normal male reference DNA labelled with biotin (green). Red bars indicate overexpression along various chromosomes, whereas green bars indicate underexpression. The profiles below show the red to green intensities along chromosomes obtained from at least 10 metaphases. (C) Comparison between EGH (right) and comparative genomic hybridisation (left) profiles and chromosome paints from chromosomes 2, 3, and 8 of the Colo320 cell line and chromosome 14 of the T47D cell line, in that order from top to bottom. Notice the overexpressed sequences hybridising to the chromosome 2q24–q33 region (right), which is not amplified (left). F Al-Mulla et al. Mol Path 2003;56:210-217 ©2003 by BMJ Publishing Group Ltd and Association of Clinical Pathologists