In Vivo Formation of Maillard Reaction Free Radicals in Mouse Skin Roger V. Lloyd, Anna J. Fong  Journal of Investigative Dermatology  Volume 117, Issue 3, Pages 740-742 (September 2001) DOI: 10.1046/j.0022-202x.2001.01448.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 ESR spectra of mouse skin treated with DHA. (a) ESR spectrum of mouse skin treated in vivo with DHA, 48 h after application, five scans averaged: (b) ESR spectrum of untreated mouse skin, five scans averaged; (c) ESR spectrum of mouse skin treated ex vivo with DHA in phosphate buffer, after 8 h at 37°C; (d) ESR spectrum of mouse skin treated ex vivo with phosphate buffer alone, after 8 h at 37°C. ESR parameters: scan range 100 Gauss; scan time 2 min; modulation amplitude 2.0 Gauss; time constant 0.25 s; microwave power 20 mW; one scan except as noted. Journal of Investigative Dermatology 2001 117, 740-742DOI: (10.1046/j.0022-202x.2001.01448.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 ESR signal intensity in ex vivo mouse skin as a function of DHA concentration in buffer: •, signal intensity after 4 h at 37°C; ▪, signal intensity after 21 h at 37°C. Journal of Investigative Dermatology 2001 117, 740-742DOI: (10.1046/j.0022-202x.2001.01448.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions