Stephanie M. Nichols, Ph. D. , Lynette Gierbolini, D. V. M. , Janis A

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Presentation transcript:

Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta Stephanie M. Nichols, Ph.D., Lynette Gierbolini, D.V.M., Janis A. Gonzalez-Martinez, Ph.D., Barry D. Bavister, Ph.D. Fertility and Sterility Volume 93, Issue 5, Pages 1591-1600 (March 2010) DOI: 10.1016/j.fertnstert.2008.12.141 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Chromosome spreads of metaphase II rhesus macaque oocytes representing (A) a normal karyotype (21 chromosomes) and (B) hyperhaploid karyotype (22 chromosomes). Bar represents 10 μm. Fertility and Sterility 2010 93, 1591-1600DOI: (10.1016/j.fertnstert.2008.12.141) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Merged immunofluorescent images of oocyte spindles and chromosomes. (A, B) Samples obtained from Regimen A cycles (full stimulation, r-FSH and r-hCG) in young females, that is, IVO oocytes. (A) Normal metaphase II oocyte displaying barrel-shaped spindle and chromosomes aligned on the spindle equator. In contrast, spindle and chromosomes in the polar body are disorganized. (B) Metaphase II oocyte that appears to display unorganized chromosome alignment around the spindle equator in a two-dimensional image. Use of confocal microscopy provided a three-dimensional image demonstrating that this oocyte was actually normal in both spindle morphology and chromosome alignment. (C) Sample obtained from a Regimen B cycle (FSH-only) from a geriatric female, that is, IVM oocyte. Spindle was malformed with a tear-drop shape (seen in confocal three-dimensional image) with evidence of chromosome lag (arrows). The abbreviation “pb” indicates polar body. Bar represents 10 μm. Fertility and Sterility 2010 93, 1591-1600DOI: (10.1016/j.fertnstert.2008.12.141) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions