Carrot allergy: Double-blinded, placebo-controlled food challenge and identification of allergens Barbara K. Ballmer-Weber, MDa, Brunello Wüthrich, MDa,

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Presentation transcript:

Carrot allergy: Double-blinded, placebo-controlled food challenge and identification of allergens Barbara K. Ballmer-Weber, MDa, Brunello Wüthrich, MDa, Andrea Wangorsch, BScb, Kay Fötisch, PhDb, Friedrich Altmann, PhDc, Stefan Vieths, PhDb Journal of Allergy and Clinical Immunology Volume 108, Issue 2, Pages 301-307 (August 2001) DOI: 10.1067/mai.2001.116430 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Immunoblot of carrot extract tested with patients’ sera (1-20, DBPCFC-positive; 21, nonresponder with a positive open challenge; 22-24, nonresponders with negative open challenge). Journal of Allergy and Clinical Immunology 2001 108, 301-307DOI: (10.1067/mai.2001.116430) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Immunoblot inhibition with carrot extract tested with sera of patients 14 (A ) and 15 (B ). Lanes: 1, carrot extract; 2, milk protein; 3, bromelain glycopeptide MUXF3; 4, fibrin glycopeptide MM; 5, rBet v 1; 6, rBet v 2; 7, birch pollen extract; 8, rApi g 1; 9, rApi g 4; 10, mugwort pollen extract; 11, no inhibitor. Inhibitor concentrations (in micrograms of protein): extracts, 100; allergens and glycans, 15. Journal of Allergy and Clinical Immunology 2001 108, 301-307DOI: (10.1067/mai.2001.116430) Copyright © 2001 Mosby, Inc. Terms and Conditions