Distribution of replicating rDNA in the WT during S phase.

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Distribution of replicating rDNA in the WT during S phase. Distribution of replicating rDNA in the WT during S phase. DAPI (cyan/blue), rDNA (AF594, red) and EdU (AF488, green) images are shown. In the DAPI and rDNA overlay (first column) and rDNA (second column) channels, maximum intensity projections of selected SIM z-stacks are shown; the remaining images represent selected slices of SIM z-stacks (1,2,3) as shown in the schematic. Arrowheads point to the chromocentres. Pie charts represent the distribution of replicating rDNA (grey-white, grey-cyan overlay) inside (white) or outside (cyan) the nucleolus based on signal quantification. Small oval extranuclear objects seen only in DAPI channel do not belong to the nuclei and likely represent bacterial contamination. (A) Very early S phase showing no or very little rDNA replication (yellow). (B) Early S and early to mid (E/Mid) S patterns. (C) Arrowheads point to the chromocentres labelled by EdU during the late S phase and forming a typical speckled pattern. Martina Dvořáčková et al. J Cell Sci 2018;131:jcs202416 © 2018. Published by The Company of Biologists Ltd