Volume 6, Issue 4, Pages (July 2013)

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Volume 6, Issue 4, Pages 1369-1372 (July 2013) Arabinosylation of a Yariv-Precipitable Cell Wall Polymer Impacts Plant Growth as Exemplified by the Arabidopsis Glycosyltransferase Mutant ray1 Sascha Gille, Vaishali Sharma, Edward E.K. Baidoo, Jay D. Keasling, Henrik Vibe Scheller, Markus Pauly Molecular Plant Volume 6, Issue 4, Pages 1369-1372 (July 2013) DOI: 10.1093/mp/sst029 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 (A) Monosaccharide compositional analysis of a buffer-extracted and ethanol-precipitated fraction of etiolated seedlings. Error bars represent the standard deviation; stars indicate significant changes to the wild-type (n = 5, p ≤ 0.05; Student’s t-test). (B) Monosaccharide compositional analysis of a buffer extract and ethanol precipitate from etiolated seedlings followed by Yariv precipitation to enrich AGP. Error bars represent the standard deviation; stars indicate significant changes to the wild-type (n = 5, p ≤ 0.05; Student’s t-test). (C) Glycosidic linkage analysis of a buffer-extracted and ethanol-precipitated fraction of etiolated seedlings. ± Standard deviation. Significant differences to wild-type (WT) Col-0 given in bold (n = 4, p ≤ 0.05; Student’s t-test). (D) In vitro Arabinosyltransferase activity of recombinant RAY1 protein with UDP–Araf as donor and methyl-β-D-galactoside as acceptor at varying acceptor concentrations. The products were analyzed by ESI–MS/TOF (Supplemental Figure 4). Error bars represent the standard deviation (n = 3). P19 control, tobacco transformation only with gene silencing suppressor P19; RAY1, transformation with RAY1 and P19. (E) Root growth phenotype of 10-day-old mutant and WT seedlings grown on vertical MS agar plates. Bar = 1 cm. Molecular Plant 2013 6, 1369-1372DOI: (10.1093/mp/sst029) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions