ONC201 activates the ISR. ONC201 activates the ISR. (A) Western blotting analysis for ATF4, CHOP, ATF3, and TRB3 on lysates from HCT116 cells cultured.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

Δ-Tocotrienol treatment is more effective against hypoxic tumor cells than normoxic cells: potential implications for cancer therapy Akira Shibata, Kiyotaka.

SCC244 significantly inhibited c-Met–driven tumor growth in cancer CDX models. SCC244 significantly inhibited c-Met–driven tumor growth in cancer CDX models.

Inhibition of CR HCT-116 (A and B) or CR HT-29 cells (B) xenografts in EPA and/or FuOx-treated SCID mice. Inhibition of CR HCT-116 (A and B) or CR HT-29.

Augmented epithelial multidrug resistance–associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A4 Ilaria Gori, Ph.D., Yoima.

Volume 120, Issue 7, Pages (June 2001)

Treatment with BLU9931 leads to tumor regression in the FGF19-overexpressing PDX-derived xenograft LIXC012. Treatment with BLU9931 leads to tumor regression.

Inhibition of ILK by QLT-0267 reduces β-catenin accumulation and inhibits Snail1 expression in obstructive nephropathy. Inhibition of ILK by QLT-0267 reduces.

Sarah A. Best, Amy N. Nwaobasi, Chrysalyne D. Schmults, Matthew R

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Sarah A. Best, Amy N. Nwaobasi, Chrysalyne D. Schmults, Matthew R

Fig. 6. Combinatorial VCPI and OV M1 treatment is efficacious in vivo and ex vivo. Combinatorial VCPI and OV M1 treatment is efficacious in vivo and ex.

S534 phosphorylation affects DNA binding and gene expression by NF-κB at late time points through regulation of p65 stability. S534 phosphorylation affects.

BV6 increases tumor burden in bone.

Fig. 1 ZIKV-related flaviviruses cause fetal demise in mice that can be prevented by mAb treatment. ZIKV-related flaviviruses cause fetal demise in mice.

Inhibition of FGFR signaling and tumor growth in SNU-16 xenograft model by administration of E7090. Inhibition of FGFR signaling and tumor growth in SNU-16.

CHK1 downregulation upon ERG overexpression.

Treatment with BLU9931 leads to tumor regression in the FGF19-amplified Hep 3B liver cancer model. Treatment with BLU9931 leads to tumor regression in.

Inhibition of KLF4 by Statins Reverses Adriamycin-Induced Metastasis and Cancer Stemness in Osteosarcoma Cells Yangling Li, Miao Xian, Bo Yang, Meidan.

Molecular Therapy - Nucleic Acids

Molecular Therapy - Nucleic Acids

Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Increase in beige/brown adipocyte characteristics.

Blockade of EDN3/EDNRB signaling impairs GSC self-renewal, cell migration, and tumorigenic capacity. Blockade of EDN3/EDNRB signaling impairs GSC self-renewal,

In vivo efficacy of Olaparib in PTEN deficient xenografts.

Overexpression of HGF decreases MET protein levels.

Allele-Specific p53 Mutant Reactivation

MEK inhibitor, U0126, attenuates cisplatin-induced renal injury by decreasing inflammation and apoptosis Sang-Kyung Jo, Won Yong Cho, Su Ah Sung, Hyoung.

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

Differentiation of AZD4785 from MAPK pathway inhibitors in vitro

IL-6 signaling affects response to erlotinib in head and neck (HNSCC) cells. IL-6 signaling affects response to erlotinib in head and neck (HNSCC) cells.

The Expression of MicroRNA-598 Inhibits Ovarian Cancer Cell Proliferation and Metastasis by Targeting URI Feng Xing, Shuo Wang, Jianhong Zhou Molecular.

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

by Emilie Clement, Hiroyuki Inuzuka, Naoe T

GR cells are dependent upon sustained CDC25C signaling as pharmacologic or genetic inhibition of CDC25C induce synthetic lethality. GR cells are dependent.

HDAC inhibitor induces RAG transcription in sensory neurons.

Fig. 3 Proteomic analysis and Western blot analysis of protein cargos of various EVs. Proteomic analysis and Western blot analysis of protein cargos of.

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

Fig. 4 Activation of mitochondrial apoptosis pathway by n-HA.

Subcutaneous tumor growth was significantly increased in Ogt-Tg/+ mice

Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model in vivo. Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model.

The SFKs confer BRAF inhibitor resistance in BRAF-mutant melanoma cells. The SFKs confer BRAF inhibitor resistance in BRAF-mutant melanoma cells. A, phospho-protein.

Active AR signaling in enzalutamide-resistant xenograft tumors.

Modeling chemotherapy-induced stress to identify rational combination therapies in the DNA damage response pathway by Ozan Alkan, Birgit Schoeberl, Millie.

Pharmacological targeting of CDs promotes response to KRASG12C inhibition in vivo. Pharmacological targeting of CDs promotes response to KRASG12C inhibition.

by Gonghua Huang, Yanyan Wang, Peter Vogel, and Hongbo Chi

Compensatory metabolic pathways during long-term selegiline treatment

Pharmacodynamic evaluation of VT-464 and ABI in MR49F xenograft model.

Targeting p53-dependent stem cell loss for intestinal chemoprotection

CRA inhibits the growth of human tumor xenografts in vivo.

NT157 treatment inhibits LNCaP xenograft growth and delays castration-resistant progression. NT157 treatment inhibits LNCaP xenograft growth and delays.

Fig. 4 ID1 is a direct CREB target.

Higher-ordered oligomeric α-synuclein induces complex morphofunctional activation of microglia. Higher-ordered oligomeric α-synuclein induces complex morphofunctional.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

The channel-kinase TRPM7 regulates antigen gathering and internalization in B cells by Mithunah Krishnamoorthy, Laabiah Wasim, Fathima Hifza Mohamed Buhari,

Effect of CDV on human SF7796 xenografts in vivo.

Comparison of in vivo activity of 4D5scFvZZ and 4D5scFv.

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

AV3 potentiates the effect of gemcitabine in the PDX model in mice

Fig. 6. Combinatorial VCPI and OV M1 treatment is efficacious in vivo and ex vivo. Combinatorial VCPI and OV M1 treatment is efficacious in vivo and ex.

Fig. 1 TLR2 expression is induced during OIS.

Fig. 2. Col IV–Ac2-26 NPs increase subendothelial collagen in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26 NPs increase subendothelial.

FGFR2 amplification in primary human gastric tumors predicts for response to NVP-BGJ398. FGFR2 amplification in primary human gastric tumors predicts for.

In vivo effect of KIN-193 on PTEN-deficient tumors.

Fig. 5 DDO-5936 dose-dependently impairs the growth of xenografted HCT116 cells in nude mice. DDO-5936 dose-dependently impairs the growth of xenografted.

Expression of PKM2 Y105F mutant in H1299 cells leads to decreased proliferation under hypoxic conditions, increased oxidative phosphorylation and reduced.

PD and efficacy of AZD4785 in a KRAS wild-type lung cancer PDX model

Presentation transcript:

ONC201 activates the ISR. ONC201 activates the ISR. (A) Western blotting analysis for ATF4, CHOP, ATF3, and TRB3 on lysates from HCT116 cells cultured with ONC201 (0 to 10 μM) for 3 to 24 hours. BFA (an ER stressor) served as a positive control. Blots are representative of two experiments. (B) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for the expression of CHOP and DR5 relative to GAPDH in HCT116 cells treated with ONC201 (10 μM) or vehicle for 24 or 48 hours. (C) Immunohistochemical analysis for CHOP in HCT116-derived subcutaneous xenografts from mice that received either vehicle (−) or ONC201 (+; 25 mg/kg). Results are representative of tissues from two vehicle-treated mice and six mice intraperitoneally or intravenously injected with ONC201 (three mice each). Scale bars, 10 μm. (D) Western blotting analysis for CHOP in lysates from colorectal cancer stem cell–like cell-derived xenografts (45) in athymic nude mice extracted 72 hours after treatment [vehicle or ONC201 (50 mg/kg, intraperitoneally)]. Tumor lysates from untreated mice (−) served as controls. (E) Densitometric analyses of the bands in the Western blots in (D). (F) qRT-PCR for the fold change in expression of CHOP and DR5 relative to GAPDH in different cell types cultured with ONC201 (compared to vehicle) for 72 hours. Data in (B), (E), and (F) are means ± SEM of three biological replicates. *P < 0.05, Student’s t test with Holm-Sidak correction, comparing ONC201-treated versus vehicle-treated cells. C. Leah B. Kline et al., Sci. Signal. 2016;9:ra18 Copyright © 2016, American Association for the Advancement of Science