Subpollen particles: Carriers of allergenic proteins and oxidases

Slides:

Advertisements

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions Kathleen R. Bartemes, BA, Kate.

Advertisements

Spleen tyrosine kinase inhibition attenuates airway hyperresponsiveness and pollution- induced enhanced airway response in a chronic mouse model of asthma

Sejal Saglani, MD, Stephen Lui, PhD, Nicola Ullmann, MD, Gaynor A

Obstructive sleep apnea syndrome and inflammation

IL-4 induces IL-13–independent allergic airway inflammation

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, MD, PhD, Yasuhiro Tabata, MD, PhD, Aaron M

Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,

Tissue factor–bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo Jin-Ah Park, PhD, Asma S.

Robert J. Snelgrove, PhD, Lisa G

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Extracellular eosinophilic traps in association with Staphylococcus aureus at the site of epithelial barrier defects in patients with severe airway inflammation

Distinct TLR-mediated pathways regulate house dust mite–induced allergic disease in the upper and lower airways Ji-Hwan Ryu, PhD, Jung-Yeon Yoo, BS,

Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring Phoebe K. Richgels, MS, Amnah.

Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model Roman Karwot, PhD, Joachim H. Maxeiner,

Innate mechanism of pollen- and cat dander–induced oxidative stress and DNA damage in the airways Koa Hosoki, MD, PhD, David Redding, MD, Toshiko Itazawa,

The airway epithelium nucleotide-binding domain and leucine-rich repeat protein 3 inflammasome is activated by urban particulate matter Jeremy A. Hirota,

Beneficial effects of high dose of L-arginine on airway hyperresponsiveness and airway inflammation in a murine model of asthma Ulaganathan Mabalirajan,

IL-33 induces innate lymphoid cell–mediated airway inflammation by activating mammalian target of rapamycin Robert J. Salmond, PhD, Ananda S. Mirchandani,

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation Si-Yen Liu, PhD, Wei-Zhi Wang,

Environmental changes could enhance the biological effect of Hop J pollens on human airway epithelial cells Seung Ihm Lee, PhD, Le Duy Pham, MD, Yoo.

Is 9 more than 2 also in allergic airway inflammation?

Thomas F. Tedder, PhD, Takashi Matsushita, MD, PhD

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model Akihiro Hasegawa, PhD, Katsuhiro.

Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production Margaret Crapster-Pregont, BS, Janice Yeo,

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

Ozone exposure induces respiratory barrier biphasic injury and inflammation controlled by IL-33 Chloé Michaudel, PhD, Claire Mackowiak, MSc, Isabelle.

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling Jennifer Kearley, PhD, Douglas S. Robinson,

Inhibition of house dust mite–induced allergic airways disease by antagonism of microRNA-145 is comparable to glucocorticoid treatment Adam Collison,

Determinants of allergenicity

Adhesion of Streptococcus pneumoniae to human airway epithelial cells exposed to urban particulate matter Naseem Mushtaq, PhD, Majid Ezzati, PhD, Lucinda.

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell–dependent murine model of allergic asthma

Spleen tyrosine kinase inhibition attenuates airway hyperresponsiveness and pollution- induced enhanced airway response in a chronic mouse model of asthma

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

Regulation of allergic airway inflammation by class I–restricted allergen presentation and CD8 T-cell infiltration James W. Wells, PhD, Christopher J.

Ozone exposure induces respiratory barrier biphasic injury and inflammation controlled by IL-33 Chloé Michaudel, PhD, Claire Mackowiak, MSc, Isabelle.

Cold temperature induces mucin hypersecretion from normal human bronchial epithelial cells in vitro through a transient receptor potential melastatin.

Allergen-induced IL-6 trans-signaling activates γδ T cells to promote type 2 and type 17 airway inflammation Md Ashik Ullah, MPharm, Joana A. Revez,

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

The Editors' Choice Journal of Allergy and Clinical Immunology

Src homology 2 domain–containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung Sun-Young Oh, PhD,

A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines David Walgraffe, PhD, Christel Mattéotti, PhD, Mohamed el Bakkoury, PhD,

What is an “eosinophilic phenotype” of asthma?

Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen Lucia Gabriele, BS, Giovanna Schiavoni, BS, Fabrizio.

Cockroach allergens: Coping with challenging complexity

Autophagy: Nobel Prize 2016 and allergy and asthma research

Mechanism of Siglec-8–mediated cell death in IL-5–activated eosinophils: Role for reactive oxygen species–enhanced MEK/ERK activation Gen Kano, MD, PhD,

Inhibiting pollen reduced nicotinamide adenine dinucleotide phosphate oxidase–induced signal by intrapulmonary administration of antioxidants blocks allergic.

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity Iris Bellinghausen, PhD, Sebastian.

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

Protective effect of Schistosoma mansoni infection on allergic airway inflammation depends on the intensity and chronicity of infection Hermelijn H.

Stephanie A. Shore, PhD, Raya D

Acute Pseudomonas challenge in cystic fibrosis mice causes prolonged nuclear factor- κB activation, cytokine secretion, and persistent lung inflammation

Isocyanate vapor-induced antigenicity of human albumin

Rhinovirus infection interferes with induction of tolerance to aeroantigens through OX40 ligand, thymic stromal lymphopoietin, and IL-33 Amit K. Mehta,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Thunderstorm-related asthma attacks

Zhenying Nie, PhD, Cole S. Nelson, PhD, David B. Jacoby, MD, Allison D

Eosinophil and neutrophil extracellular DNA traps in human allergic asthmatic airways Ryszard Dworski, MD, PhD, Hans-Uwe Simon, MD, PhD, Aimee Hoskins,

The extra domain A of fibronectin is essential for allergen-induced airway fibrosis and hyperresponsiveness in mice Martin Kohan, MSc, Andres F. Muro,

Macrolide antibiotics and asthma treatment

The effects of gastric digestion on codfish allergenicity

Natural history of cow’s milk allergy

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Chitinases and chitinase-like proteins in TH2 inflammation and asthma

TNF can contribute to multiple features of ovalbumin-induced allergic inflammation of the airways in mice Susumu Nakae, PhD, Carolina Lunderius, PhD,

Presentation transcript:

Subpollen particles: Carriers of allergenic proteins and oxidases Attila Bacsi, PhD, Barun K. Choudhury, PhD, Nilesh Dharajiya, MD, Sanjiv Sur, MD, Istvan Boldogh, PhD Journal of Allergy and Clinical Immunology Volume 118, Issue 4, Pages 844-850 (October 2006) DOI: 10.1016/j.jaci.2006.07.006 Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 Release of SPPs from ragweed pollen grains. A, Real-time microscopic images of SPPs release from ragweed pollen grains. Left to right, Vesicle-like formations on the pollen surface (magnification ×100); the release of 0.5 to 4.5 μM of SPPs (5, 10, and 15 minutes). B, Size distributions of ragweed pollen grains 5 minutes (shaded panel) and 30 minutes (nonshaded panel) after hydration. FSC, Forward scatter parameter. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Release of SPPs requires NAD(P)H oxidase activity. A, Formazan formation at the site of SPP release (ragweed pollen). B and C, Ragweed (Fig 2, B) and pigweed (Fig 2, C) pollen grains oxidize H2DCF-DA into fluorescent DCF. D, Percentage of the DCF-positive pollen particles (open columns) releasing SPPs (solid columns). E, Inhibition of SPP formation. Open columns, Ragweed; solid columns, pigweed. All results are presented as means ± SEM (n = 4-7). ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001 versus control. MT, Mock treated. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 SPPs possess redox activity. A, Ragweed SPPs showing DCF fluorescence (magnification ×196). B, SPP-mediated O2·− generation is inhibited by NAD(P)H oxidase inhibitors or heat inactivation. SOD, Superoxide dismutase; CAT, catalase; GO, glucose oxidase. All results are presented as means ± SEM (n = 4-7). C, In-gel NBT reduction by proteins in SPPs after nondenaturing PAGE. Lane 1, SPP; lane 2, heat-treated SPP; lane 3, RWE; lane 4, heat-treated RWE. ∗∗∗∗P < .0001 versus control. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 4 SPPs from ragweed pollen grains contain Amb a 1. A, SDS-PAGE analysis of RWE and lysates of SPPs. The SPPs were released from the pollen grains and then lysed and subjected to SDS-PAGE. Fractionated proteins were stained with Coomassie brilliant blue G250. B, Amb a 1 in SPPs shown by means of Western blot analysis. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 5 SPPs increase levels of ROS in airway epithelial cells. A, NHBE cells grown in submerged (solid columns) or air-liquid interphase cultures (open columns) treated with ragweed SPP. B, A549 cells exposed to ragweed or pigweed SPPs. Glucose oxidase (GO) was used as a positive control. MT, Mock treated; NAC, N-acetyl-L-cysteine. All results are presented as means ± SEM of 4 to 6 measurements. ∗∗∗∗P < .0001 versus control. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 6 A and B, SPPs induce airway inflammation. Sensitized mice were challenged with PBS, Amb a 1, or SPPs with (solid columns) or without (open columns) QA. Eosinophils (Fig 6, A) and total inflammatory cells (Fig 6, B) in BAL fluids are shown (± SEM; n = 3-5). C, Inflammatory cell infiltration in the peribronchial area (upper row) and goblet cell metaplasia (lower row). Representative images of serial lung sections are shown (n = 7-9). ∗∗∗P < .001; ∗∗∗∗P < .0001 versus control. Journal of Allergy and Clinical Immunology 2006 118, 844-850DOI: (10.1016/j.jaci.2006.07.006) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions