1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression in Skh:hr1 mouse skin. 1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression.

Slides:

Advertisements

Similar presentations

Jing-Yi Lin, MDa,. , M. Angelica Selim, MDa, Christopher R. Shea, MDb,

Advertisements

Inflammatory Murine Skin Responses to UV-B Light Are Partially Dependent on Endothelin-1 and Mast Cells Martin Metz, Verena Lammel, Bernhard F. Gibbs,

Topical Application of A Novel Immunomodulatory Peptide, RDP58, Reduces Skin Inflammation in the Phorbol Ester-Induced Dermatitis Model Christopher G.

Tracking angiogenesis induced by skin wounding and contact hypersensitivity using a Vegfr2-luciferase transgenic mouse by Ning Zhang, Zuxu Fang, Pamela.

The protective effect of sunscreens, vitamin E 6% cream, and betamethasone 0.1% cream on solar-simulating radiation-induced erythema and neutrophil influx

Photobiologic and Photoimmunologic Characteristics of XPA Gene-Deficient Mice Takeshi Horio, Hiroko Miyauchi-Hashimoto, Kazue Kuwamoto, Satoshi Horiki,

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Hee-Young Park, PhD, Jin Lee, Sameer Kapasi, Shaun Peterson, Barbara A

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Shobhan Gaddameedhi, Christopher P. Selby, Michael G

Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent manner. Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent.

[Nle4-D-Phe7]-α-Melanocyte-Stimulating Hormone Significantly Increased Pigmentation and Decreased UV Damage in Fair-Skinned Caucasian Volunteers Ross.

Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin

Effects of SC144 on in vivo ovarian tumor.

The Aryl Hydrocarbon Receptor Is Involved in UVR-Induced Immunosuppression Fatemeh Navid, Anika Bruhs, Winfried Schuller, Ellen Fritsche, Jean Krutmann,

Langerhans Cells Are Required for UVR-Induced Immunosuppression

Fig. 4. Antitumor efficacy of ERY974 against various cancer types.

Ultraviolet Modulation of Human Macrophage Metalloelastase in Human Skin In Vivo Jin Ho Chung, Jin Young Seo, Mi Kyoung Lee, Hee Chul Eun, Joo Heung Lee,

Single UVB Overexposure Stimulates Melanocyte Proliferation in Murine Skin, in Contrast to Fractionated or UVA-1 Exposure Arne van Schanke, Marjan J.

Epidermal COX-2 Induction Following Ultraviolet Irradiation: Suggested Mechanism for the Role of COX-2 Inhibition in Photoprotection Catherine S. Tripp,

Opening of Chloride Channels by 1α,25-Dihydroxyvitamin D3 Contributes to Photoprotection against UVR-Induced Thymine Dimers in Keratinocytes Vanessa.

P38 Mitogen-Activated Protein Kinase Inhibitor Protects the Epidermis Against the Acute Damaging Effects of Ultraviolet Irradiation by Blocking Apoptosis.

Vitamin A Exerts a Photoprotective Action in Skin by Absorbing Ultraviolet B Radiation Christophe Antille, Christian Tran, Olivier Sorg, Pierre Carraux,

Elevation of Intracellular Cyclic AMP in Alloreactive CD4+ T Cells Induces Alloantigen- Specific Tolerance That Can Prevent GVHD Lethality In Vivo Matthew.

Sirolimus Reduces the Incidence and Progression of UVB-Induced Skin Cancer in SKH Mice even with Co-administration of Cyclosporine A Brian C. Wulff,

Topical Imiquimod Treatment Prevents UV-Light Induced Loss of Contact Hypersensitivity and Immune Tolerance Thomas H. Thatcher, Irina Luzina, Rita Fishelevich,

XPA Gene-Deficient, SCF-Transgenic Mice with Epidermal Melanin Are Resistant to UV- Induced Carcinogenesis Fumikazu Yamazaki, Hiroyuki Okamoto, Hiroko.

Protection against UVR Involves MC1R-Mediated Non-Pigmentary and Pigmentary Mechanisms In Vivo Samantha Robinson, Sandra Dixon, Suzannah August, Brian.

Tej Pratap Singh, Gerlinde Mayer, Peter Wolf

The Human Hair Follicle: A Reservoir of CD40+ B7-Deficient Langerhans Cells that Repopulate Epidermis After UVB Exposure Anita C. Gilliam, Inger B. Kremer,

Attenuation of UVB-Induced Sunburn Reaction and Oxidative DNA Damage with no Alterations in UVB-Induced Skin Carcinogenesis in Nrf2 Gene-Deficient Mice

1,25-Dihydroxyvitamin D Exerts Similar Immunosuppressive Effects as UVR but Is Dispensable for Local UVR-Induced Immunosuppression Agatha Schwarz, Fatemeh.

PKCε Overexpression, Irrespective of Genetic Background, Sensitizes Skin to UVR- Induced Development of Squamous-Cell Carcinomas Jordan M. Sand, Moammir.

A Bio-Mimetic Approach to DNA Photoprotection

Urocanic Acid: An Endogenous Regulator of Langerhans Cells

Interdependence between Heme Oxygenase-1 Induction and Estrogen-Receptor-β Signaling Mediates Photoimmune Protection by UVA Radiation in Mice Vivienne.

Infrared Radiation Confers Resistance to UV-Induced Apoptosis Via Reduction of DNA Damage and Upregulation of Antiapoptotic Proteins Christian Jantschitsch,

IL-23 Antagonizes UVR-Induced Immunosuppression through Two Mechanisms: Reduction of UVR-Induced DNA Damage and Inhibition of UVR-Induced Regulatory T.

RXRα Ablation in Epidermal Keratinocytes Enhances UVR-Induced DNA Damage, Apoptosis, and Proliferation of Keratinocytes and Melanocytes Zhixing Wang,

C/EBPα Expression Is Downregulated in Human Nonmelanoma Skin Cancers and Inactivation of C/EBPα Confers Susceptibility to UVB-Induced Skin Squamous Cell.

Formation of Antigenic Quinolone Photoadducts on Langerhans Cells Initiates Photoallergy to Systemically Administered Quinolone in Mice Akihiro Ohshima,

Therapy-induced senescence of primary cells.

Ultraviolet A Irradiation of C57BL/6 Mice Suppresses Systemic Contact Hypersensitivity or Enhances Secondary Immunity Depending on Dose Scott N. Byrne,

Possible Involvement of Gelatinases in Basement Membrane Damage and Wrinkle Formation in Chronically Ultraviolet B-exposed Hairless Mouse Shinji Inomata,

Comparison of the percentages of ducts in the pancreas containing cells showing positive staining for the different markers between control hamster Ig–and.

Upon challenge with oxazolone, human leukocytes (mainly consisting of T cells) infiltrate the dermis and epidermis. Upon challenge with oxazolone, human.

Skin-Infiltrating Monocytes/Macrophages Migrate to Draining Lymph Nodes and Produce IL-10 After Contact Sensitizer Exposure to UV-Irradiated Skin Eiko.

Photoimmunoprotection by UVA (320–400nm) Radiation Is Determined by UVA Dose and Is Associated with Cutaneous Cyclic Guanosine Monophosphate Munif Allanson,

Modulation of IL-10, IL-12, and IFN-γ in the Epidermis of Hairless Mice by UVA (320– 400 nm) and UVB (280–320 nm) Radiation Jie Shen, Shisan Bao, Vivienne.

Fig. 5 Treatment with BMS (PO BID) protects from wasting and colitis in two SCID mouse models. Treatment with BMS (PO BID) protects from.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Quercetin induces arrest in G1 phase of cell cycle in P39 xenografts.

Quercetin induces apoptosis in P39 xenografts.

A, TSG-6 staining in TRAMP tissue and TRAMP cell lines.

Anti-Flk-1 mAb inhibits vascularization of Matrigel plugs.

Suppression by nobiletin of the TPA-induced increase in PCNA-positive cells in mouse epidermis. Suppression by nobiletin of the TPA-induced increase in.

Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

HMQ1611 inhibited breast tumor growth in mice.

YH25448 less suppresses WT EGFR than osimertinib in vivo.

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Effects of UVB on AKT in SKH-1 mouse epidermis.

Acute phenotypes of HPV16 E6/E7 transgenic mouse anal epithelium.

Lapatinib cooperates with PLX4032 to inhibit BRAF-mutant thyroid cancer cell growth. Lapatinib cooperates with PLX4032 to inhibit BRAF-mutant thyroid cancer.

Effect of MZ treatment on lung colony formation in an experimental metastasis. Effect of MZ treatment on lung colony formation in an experimental metastasis.

Effects of ZOL treatment on pulmonary metastases.

BME treatment increases cytotoxic activity of NK3

Recruitment of CD8+, CD4+, and Foxp3+ cells into oral lesions in response to anti–PD-1 treatment. Recruitment of CD8+, CD4+, and Foxp3+ cells into oral.

Fig. 3 MCA-mediated neutrophil recruitment accelerates bacterial clearance in the skin. MCA-mediated neutrophil recruitment accelerates bacterial clearance.

Expression of the angiogenic phenotype in the 4-NQO mouse model of HNSCC. A, tissue sections containing areas of normal, hyperkeratosis, dysplasia, and.

Parthenolide inhibits tumor promotion and increases p21 expression in vivo. Parthenolide inhibits tumor promotion and increases p21 expression in vivo.

Presentation transcript:

1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression in Skh:hr1 mouse skin. 1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression in Skh:hr1 mouse skin. Mice were exposed to 1 × 3 MEDs of solar-simulated UVR (3.98 kJ/m2 UVB and 63.8 kJ/m2 UVA) and were treated topically on the UV-irradiated dorsal surface immediately after UVR exposure with 100 μL of vehicle only, 1,25(OH)2D3, or JN. A, histology: skin biopsies were collected and fixed 3 hours after UVR exposure for immunohistochemical and image analysis to quantify positively stained nuclei (arrows) as a proportion of total nuclei. Darkly stained nuclei (arrows) indicate the presence of CPD (thymine dimers). i, negative control; ii, vehicle; iii, vehicle + UVR; iv, 1,25(OH)2D3 + UVR; and v, JN + UVR. B, image analysis results of 3 hours post–UVR skin biopsies presented as the mean of 3 skin sections ± 1 SD. Area of positively stained nuclei expressed as the percentage of area of total nuclei. Significantly different from vehicle-treated UV-irradiated mice. P < 0.01, n = 3. C, skin biopsies were taken 24 hours after UVR exposure for routine H&E staining. Sunburn cells were identified under a light microscope as cells with pyknotic nuclei surrounded by an eosinophilic cytoplasm and were counted along the length of each skin section. Results presented as the mean number of sunburn cells per 1,000 linear micrometer of skin section; n = 3 (mice per group). Significantly different from vehicle: **, P < 0.01; n = 3. D, edema was measured as dorsal skin-fold thickness in the mice 48 hours after UVR exposure. Significantly different from vehicle-treated UV-irradiated mice. **, P < 0.01; n = 5. E, mice were sensitized on nonirradiated abdominal skin 7 and 8 days after UVR exposure with 2% oxazolone and then challenged on their ears 7 days after sensitization and ear swelling recorded 16 hours later. Results were calculated as the difference between pre- and postchallenge ear thickness measurements of nonirradiated mice as a proportion of the difference between pre- and postchallenge ear thickness measurements of irradiated mice for each treatment. Immunosuppression expressed as 100% minus this value ± SEM. ***, P < 0.001 significantly different from vehicle; ###, P < 0.001 significantly different from 1,25(OH)2D3 11.4 ρmol/cm2 and JN 22.8 ρmol/cm2; n = 5. Katie M. Dixon et al. Cancer Prev Res 2011;4:1485-1494 ©2011 by American Association for Cancer Research