Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

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Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells. Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells. A. Allopurinol up-regulates DR5 protein expression. PC-3 and DU145 cells were treated with allopurinol at the indicated concentrations for 24 h. CT, treated with DMSO (control). Arrows indicate DR5 proteins. Asterisk indicates nonspecific band. The 48- and 43-kDa bands correspond to long and short DR5 isoforms, respectively. B. Allopurinol up-regulates DR5 mRNA expression. PC-3 and DU145 cells were treated with allopurinol at the indicated concentrations for 24 h. Northern blotting was done as described in Materials and Methods. Ethidium bromide–stained 28S and 18S rRNA are shown as controls. CT, treated with DMSO (control). C. Allopurinol (ALLO) enhanced DR5 promoter activity. Luciferase assay was carried out with PC-3 and DU145 cells treated with 200 μmol/L allopurinol for 24 h after transfection of a luciferase plasmid containing DR5 promoter (pDR5PF). pGVB2 is a vacant control plasmid. Columns, mean of triplicate experiments; bars, SD. *, P < 0.05. RLU, relative luciferase units. Takashi Yasuda et al. Mol Cancer Res 2008;6:1852-1860 ©2008 by American Association for Cancer Research