Compensatory hyperinsulinemia maintains euglycemia in HFHC-fed guinea pigs but is lost with STZ treatment. Compensatory hyperinsulinemia maintains euglycemia.

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Compensatory hyperinsulinemia maintains euglycemia in HFHC-fed guinea pigs but is lost with STZ treatment. Compensatory hyperinsulinemia maintains euglycemia in HFHC-fed guinea pigs but is lost with STZ treatment. (A) Insulin was detected by direct ELISA in serum from fasted guinea pigs fed a normal diet (n=16), HFHC diet (n=18) or HFHC diet and treated with STZ (HFHC/STZ, n=18). Fasting hyperinsulinemia is present in guinea pigs after 8 weeks of consuming the HFHC diet. Treatment of HFHC-fed guinea pigs with STZ leads to impairment of the compensatory insulin response; however, insulin production is retained in HFHC/STZ guinea pigs at a level comparable to normal-diet controls. ***P<0.001, one-way ANOVA. (B) Fasting blood glucose concentrations were measured from fasting samples taken simultaneously for insulin quantification by direct ELISA. Fasting hyperglycemia is absent in the presence of compensatory hyperinsulinemia in HFHC-fed guinea pigs. Fasting hyperglycemia is present in HFHC/STZ guinea pigs after impairment of the compensatory insulin response. ***P<0.001, one-way ANOVA. (C) Glucose-stimulated insulin secretion performed in guinea pigs after 8 weeks of HFHC feeding, then repeated 21 days after receiving STZ. Residual function is retained in β cells after STZ treatment (n=3). *P<0.05, paired one-way ANOVA. Brendan K. Podell et al. Dis. Model. Mech. 2017;10:151-162 © 2017. Published by The Company of Biologists Ltd