Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion fibroblasts  Natalie N. Rizk, Ph.D., Ghassan M. Saed, Ph.D., Michael P. Diamond, M.D.  Fertility and Sterility  Volume 86, Issue 4, Pages 1217-1222 (October 2006) DOI: 10.1016/j.fertnstert.2006.04.022 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Real-time PCR of the insulin receptor after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility 2006 86, 1217-1222DOI: (10.1016/j.fertnstert.2006.04.022) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Real-time PCR of the IGF-1 receptor after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility 2006 86, 1217-1222DOI: (10.1016/j.fertnstert.2006.04.022) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Real-time PCR of IGF-1 after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts, (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Sample size, three patients (mean ± SEM; *P<.05 compared with column A, #P<.05 compared with column A′; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility 2006 86, 1217-1222DOI: (10.1016/j.fertnstert.2006.04.022) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 Concentrations of IGF-1 (in pg/mL) after glucose treatment: normal peritoneal fibroblasts, (A) 100 mg/dL, (B) 350 mg/dL, (C) 550 mg/dL, and (D) 850 mg/dL and adhesion-tissue fibroblasts (A′) 100 mg/dL, (B′) 350 mg/dL, (C′) 550 mg/dL, and (D′) 850 mg/dL. Concentrations of IGF-I were measured in medium in which cells were incubated. Sample size, three patients (mean ± SEM; *P<.05 compared with normal fibroblasts at 100 mg/dL; one-way analysis of variance). Rizk. Peritoneal fibroblasts response to hyperglycemia. Fertil Steril 2006. Fertility and Sterility 2006 86, 1217-1222DOI: (10.1016/j.fertnstert.2006.04.022) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions