Example of a putative bridging fiber.

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Example of a putative bridging fiber. Example of a putative bridging fiber. (A) A single SBF-SEM image is shown of the area of interest, with a 3D model of microtubules and kinetochores overlaid. Opposing sister kinetochores (red and yellow) are connected to K-fibers (dark blue and light blue, respectively). (B) Sequential SEM images (above) with segmentation (below) that make up the image shown in A. Each image is separated by 60 nm in the z direction. Note that, in this example, the bridging fiber runs on the exterior of the spindle not the interior as predicted (Kajtez et al., 2016). A movie showing putative bridging fibers is shown in Movie 3. Faye M. Nixon et al. J Cell Sci 2017;130:1845-1855 © 2017. Published by The Company of Biologists Ltd