Colocalisation and influence on migration.

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Colocalisation and influence on migration. Colocalisation and influence on migration. (A) Immunostaining of cryosections from a 12.5-dpc embryo showing the area of the thoracic DRG with periostin- (green) and laminin-specific (red) antibodies. Note the substantial overlap of the expression domains, both within the DRG and along the axonal projection. (B) Immunostaining of DRG explant cultures derived from wild-type and periostin-deficient embryos (12.5 dpc) using the Neurofilament200 (Nf200, green) antibody and an antibody specific for Sox10 (red). Axonal projections (lengths and numbers) are comparable between genotypes, whereas the number of migrating Schwann cell precursors (Sox10 positive) is clearly reduced in periostin-deficient cultures. Nf200/Sox10, co-staining of neurofilaments and Sox10. Scale bars: 100 µm (A,B). Eva Sonnenberg-Riethmacher et al. J Cell Sci 2015;128:3345-3355 © 2015. Published by The Company of Biologists Ltd