Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. Ezrin in its open, activated conformation binds and colocalizes.

Slides:

Advertisements

Similar presentations

Das_Figure S1 USP15 USP4 USP11 49% 60% 39 % 71 % 52 % 54 %

Advertisements

Volume 21, Issue 16, Pages (August 2011)

IP-MS identifies FAM49B interacting protein Rac.

HURP Is a Ran-Importin β-Regulated Protein that Stabilizes Kinetochore Microtubules in the Vicinity of Chromosomes Herman H.W. Silljé, Susanna Nagel,

DEAR1 binds to SMAD3 and induces SMAD3 ubiquitination.

GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting Johannes Egerer, Denise Emmerich, Björn Fischer-Zirnsak, Wing Lee Chan, David.

The Polycomb Protein Pc2 Is a SUMO E3

Volume 24, Issue 1, Pages (January 2016)

Volume 24, Issue 15, Pages (August 2014)

NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus. NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus.

Christian Pohl, Stefan Jentsch Cell

Kindlin-1 Regulates Epidermal Growth Factor Receptor Signaling

HRTV and SFTSV NSs, but not UUKV NSs, inhibits JAK/STAT IFN signaling.

Central Spindle Assembly and Cytokinesis Require a Kinesin-like Protein/RhoGAP Complex with Microtubule Bundling Activity Masanori Mishima, Susanne Kaitna,

SYCE2 directly binds to the chromoshadow domain of HP1α.

Yuming Wang, Jennifer A. Fairley, Stefan G.E. Roberts Current Biology

Volume 12, Issue 9, Pages (September 2015)

Characterization of the Human Hair Keratin–Associated Protein 2 (KRTAP2) Gene Family Hiroki Fujikawa, Atsushi Fujimoto, Muhammad Farooq, Masaaki Ito,

Shijiao Huang, Danming Tang, Yanzhuang Wang Developmental Cell

The Role of NEDD1 Phosphorylation by Aurora A in Chromosomal Microtubule Nucleation and Spindle Function Roser Pinyol, Jacopo Scrofani, Isabelle Vernos

Lizhong Xu, Veronica Lubkov, Laura J. Taylor, Dafna Bar-Sagi

Heterochromatin Formation in Mammalian Cells

Volume 42, Issue 2, Pages e3 (July 2017)

Jacopo Scrofani, Teresa Sardon, Sylvain Meunier, Isabelle Vernos

Frida E. Kleiman, James L. Manley Cell

A Role for the Fizzy/Cdc20 Family of Proteins in Activation of the APC/C Distinct from Substrate Recruitment Yuu Kimata, Joanne E. Baxter, Andrew M.

Volume 14, Issue 12, Pages (March 2016)

Volume 13, Issue 10, Pages (May 2003)

Volume 19, Issue 14, Pages (July 2009)

ASPP2 Regulates Epithelial Cell Polarity through the PAR Complex

Volume 24, Issue 21, Pages (November 2014)

Volume 24, Issue 1, Pages (January 2016)

Hua Gao, Yue Sun, Yalan Wu, Bing Luan, Yaya Wang, Bin Qu, Gang Pei

Volume 16, Issue 4, Pages (April 2009)

Plk1 inhibition affects the NuMA turnover at the spindle pole.

Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent Hiroki Hikasa, Yoshitaka Sekido, Akira Suzuki

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Abraxas binds to BRCT–PALB2(L21P) and is involved in the recruitment of this fusion protein or of endogenous PALB2 to sites of DNA damage. Abraxas binds.

Fusion of PALB2 to the BRCT repeats of BRCA1 mediates the assembly of DNA damage foci by PALB2 and RAD51 in BRCA1-deficient cells. Fusion of PALB2 to the.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

MET/β-catenin complexes during synapse development.

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Interaction of periostin with ECM molecules.

Volume 11, Issue 19, Pages (October 2001)

14-3-3γ phosphorylated at S59 by Lats2 in response to UV damage.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

SLK/LOK-phosphorylated and activated ezrin prevents MISP localization at the cell cortex. SLK/LOK-phosphorylated and activated ezrin prevents MISP localization.

Loss of ezrin and MISP leads to the formation of cysts containing multiple lumens and spindle orientation defects. Loss of ezrin and MISP leads to the.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Fig. 2. Acetylation stiffens primary cilia.

Proteolytically processed forms of Gli2 do not localize to cilia but the activator, repressor and zinc finger domains are dispensable for the ciliary localization.

Eps8 interacts with IRTKS and is involved in the generation of dorsal morphology. Eps8 interacts with IRTKS and is involved in the generation of dorsal.

Interaction of NM-GFPagg with G3BP and p62.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Ub-binding ability of ERα.

Volume 9, Issue 1, Pages (January 2002)

Vps36 interacts with Smo in the absence of Hh

Ubiquitin mediates the interaction between Smo and Vps36.

Structure–function analysis of CGEF-1.

GOLPH3 interacts with COG complex proteins.

Exosomal transfer of sortilin to a target cell.

DAPK interacts with HSF1 in vivo and in vitro.

TRIM17 and Beclin 1 colocalize with the anti-autophagy factor Mcl-1.

Increased levels of MISP at the cell cortex promote spindle orientation defects. Increased levels of MISP at the cell cortex promote spindle orientation.

Volume 10, Issue 4, Pages (April 2017)

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Volume 41, Issue 4, Pages (February 2011)

Presentation transcript:

Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. Ezrin in its open, activated conformation binds and colocalizes with MISP at the cell cortex. (A) Flag–MISP and GFP–ezrin were co-expressed in HEK293T cells. Complexes were immunoprecipitated with Flag-M2 antibody (Flag-IP) or anti-GFP antibody (GFP-IP), and analyzed by western blotting using the indicated antibodies. (B) Co-IP of endogenous ezrin from HeLa cells as analyzed by western blotting using anti-MISP or -ezrin antibodies. IgG, control immunoglobulin. (C) Domain structure of full-length ezrin (comprising the N-terminal located FERM domain, the α-tail and the C-ERMAD domain. Indicated residue T567 gets phosphorylated, whereas R579 is important for actin binding and prevents binding between the N- and C-termini. In vitro GST pulldown (GST-PD) of recombinant GST-MISP and His-tagged ezrin constructs. Purified proteins were incubated, subjected to GST pulldown and analyzed by western blotting using the indicated antibodies. (D,E) Co-staining of MISP (magenta) and ezrin (green) antibodies in a Caco-2 BBe cyst (D) or a mitotic HeLa cell (E). DNA is stained with Hoechst 33258 (blue). Localization is either shown in a maximum projection of three planes or in a single confocal plane. Insets show higher magnification of the framed region. Scale bars: 5 µm. Yvonne T. Kschonsak, and Ingrid Hoffmann J Cell Sci 2018;131:jcs214544 © 2018. Published by The Company of Biologists Ltd