Supplementary Figure 3 Sequential Branch-off De novo * * * * ** Histology of the carcinoma Invasive carcinoma Supplementary Figure 3 Case 5 (16) H i * H Case 6 (2) H * H Case 1 H H Case 2 H Case 3 Annotation box Sequential H Gastric type Intestinal type Pancreatobiliary type Oncocytic feature Case 4 H i Case 30 H L Tubular adenocarcinoma Mucinous adenocarcinoma Case 7 H i i H, L, i, IPMN with high-grade dysplasia IPMN with low-grade dysplasia Incipient IPMN Case 26 H L *, Second metachronous invasive cancer **, Second synchronous invasive cancer //, Margin of the first operation, recurrence case (year), Time to recurrence , Contiguous neoplastic lesion between IPMN and PDA via main pancreatic duct Case 29 H L Case 18 L Case 13 L L L i KRAS WT GNAS WT G12V R201H Case 12 L L i G12D R201C G12R Q227H Case 21 (2) H * H i Q61H/Q61R G12C Case 25 A142T Branch-off L Case 8 TP53 Mutation Aberrant L SMAD4 Mutation Aberrant CDKN2A/p16 Mutation Aberrant Case 11 L i CTNNB1 mutation and/or β-catenin nuclear stain RNF43 mutation and/or loss Case 14 L L Other mutations Case 16 L (Example) TP53 CDKN2A/p16 SMAD4 Case 19 Mutation L IHC Case 24 H L L GNAS Case 23 (1) H L * KRAS Case 17 L i Case 15 L i Case 10 De novo L Case 22 L Case 28 ** L i Case 20 (4) i H L i (10) Case 27 H i H H Case 9 H IPMN subtype 1 2 3 4 5 6 7 8 9 10 Distance from invasive cancer (cm)