Flow cytometry of subcutaneous adipose tissue stromovascular cells.

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Flow cytometry of subcutaneous adipose tissue stromovascular cells. Flow cytometry of subcutaneous adipose tissue stromovascular cells. A: After excluding doublets and dead cells, a CD14 versus CD45 contour plot identified three stromovascular cell populations (PAs, LYMs, and ATMs). B: Cytospins of each population stained for CD68 confirmed that the CD14+CD45+ population represents ATMs. Scale bar = 20 μm. C: Flow cytometry phenotype of gated PA, LYM and ATM populations stained for CD11c and CD206 reveals that PA cells do not express either marker, while a minority of LYM cells express low levels of CD11c. ATM cells could be separated into three distinct subpopulations when stained for CD11c and CD206; one of these, CD11c+CD206− ATMs, had a similar phenotype to blood monocytes (MONO). Background staining by isotype control antibodies is indicated by gray lines. D: CD11c+CD206− and CD11c− ATMs were isolated by flow cytometry cell sorting and stained with Oil red O. Representative images show CD11c+CD206+ ATMs contain more lipid than CD11c− ATMs. Scale bar = 10 μm. (A high-quality digital representation of this figure is available in the online issue.)‏ John M. Wentworth et al. Diabetes 2010;59:1648-1656 ©2010 by American Diabetes Association