Expression analysis of periostin in DRG

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Expression analysis of periostin in DRG Expression analysis of periostin in DRG. (A–D) Periostin expression during development was followed by using in situ hybridisation with a 35S-labelled periostin-specific probe on sagittal sections of wild-type embryos at different stages. Expression analysis of periostin in DRG. (A–D) Periostin expression during development was followed by using in situ hybridisation with a 35S-labelled periostin-specific probe on sagittal sections of wild-type embryos at different stages. Expression in several connective tissues, the periosteum and the valves of the heart has been previously described. Also note the strong expression in the umbilical cord, highlighted with an asterisk (A,B). (A) Expression at 10.5 dpc. Note the expression in the DRG, indicated by arrows. (C) Expression at 16.5 dpc shows that the robust expression within the DRG persists (arrows). (B,D) Expression at 12.5 dpc. D is a higher magnification of the boxed area in B. Note the higher expression along axonal projections (arrowheads) compared with the expression level within the DRG (arrows). Scale bars: 250 µm (A); 500 µm (B,C); 125 µm (D). Eva Sonnenberg-Riethmacher et al. J Cell Sci 2015;128:3345-3355 © 2015. Published by The Company of Biologists Ltd