Microscope Identification Microscopy Microscope Identification

Terminology Microscopy is a technique used to increase the size of specimen. Magnification: the quality of making an image appear larger than its actual size. Resolution: a measure of the clarity of an image.

Large but blurry Large & Clear

Self Check Shantel wanted to see what her skin cells looked like under a microscope. After making a wet-mount slide, she began studying the specimen with her compound microscope. While peering through the eyepiece, Shantel noticed that the image was clear (not blurry), but was not large enough. Is Shantel having an issue with resolution or magnification? Magnification

What’s My Power? To calculate the power of magnification, multiply the power (magnification) of the eye piece by the power of the objective lens. 10 x 40 = 400 X

Practice Eye Piece x Objective Lens = Total Magnification Practice: Shakeem wanted to determine the total magnification of his microscope. He noticed that the eye piece had a magnification of 10x and the highest powered objective lens was 50x. What is the total magnification of Shakeem’s microscope? 10 x 50 = 500

Total Magnification / Eye Piece = Objective Lens More Practice Marco is using a microscope that has a total magnification of 200. What is the power of Marco’s objective lens? Total Magnification / Eye Piece = Objective Lens 200 / 10 = 20

Comparing Powers of Magnification We can see better details with higher powers of magnification, but we cannot see as much of the image.

Application Which of these images would be viewed at a higher power of magnification? How were you able to determine the correct answer? The image is larger, but less of the image can be seen.

Types of Microscopes Dissecting Microscope Light/Compound Microscope Electron Microscope

Dissecting Microscope Light reflects off the surface of the object You can see the object with the naked eye Magnifies 5x-30x

Compound Microscope Light shines through the object Objects are to small to be seen with the naked eye. Magnifies 100x-400x

Electron Microscope Uses electrons to form an image of an object Objects are too small to see with a compound microscope. i.e. viruses and bacteria Magnification power: 200,000 x

Practice Darnell wanted to see the cellular makeup of a bacterial cell. Which microscope will provide Darnell with enough magnification power to see 200,000 x greater than the naked eye to view the bacterial cell? Electron Microscope

How to Make a Wet-Mount Slide… Get a clean slide and coverslip from your teacher. Carefully place your specimen on the glass slide. Place one drop of water on top of your specimen. Slowly lower the cover slip on top of the specimen at a 45 degree angle.

Why 45 degrees? Lowering a coverslip on top of your specimen at a 45 degree angle very important! This prevents air bubbles from being trapped under the coverslip. Air bubbles make it difficult to look at your specimen.

Course Adjustment Knob Eye Piece Revolving Nose Piece Objective Lens Stage Clip Stage Diaphragm Course Adjustment Knob Light Source Fine Adjustment Knob Base