PTx pretreatment abrogates the glucagonostatic effect of high glucose concentrations. PTx pretreatment abrogates the glucagonostatic effect of high glucose.

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PTx pretreatment abrogates the glucagonostatic effect of high glucose concentrations. PTx pretreatment abrogates the glucagonostatic effect of high glucose concentrations. Islets of C57BL/6 mice were pretreated (PTx) or not (CT) with PTx (18 h with 200 ng/mL during culture). A and B: Groups of seven islets were then incubated for 1 h in the presence of MixAA 6. A: Islets were incubated in a medium without glucose (G0) and supplemented or not with 10 µmol/L SST14. Values are means ± SE for 10–12 batches of islets. **P < 0.01 for comparison between different conditions or treatments of islets. B: Islets were incubated in a medium containing various glucose concentrations (0, 1, 2, 5, 7, 15, and 30 mmol/L). Values are means ± SE for 11–14 batches of islets. *P < 0.05 and **P < 0.01 vs. the average secretion of islets in G0. C and D: Islets were perifused with MixAA 2. The glucose concentration was changed between 7 and 30 (C) or 20 (D) mmol/L (G7, G30, and G20, respectively) as indicated. Traces are means ± SE for three to four experiments with islets from different preparations. E and F: Univariate scatter plots showing individual data and means ± SE of the average glucagon secretion calculated from the experiments shown above (G7, mean of 20–40 min and 104–124 min; G20 or G30, mean of 44–96 min). *P < 0.05 for comparison between different conditions or treatments of islets. Bao-Khanh Lai et al. Diabetes 2018;67:2239-2253 ©2018 by American Diabetes Association