Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens Juan A. Asturias, PhD, Ignacio Ibarrola, PhD, Angel Ferrer,

Slides:

Advertisements

Similar presentations

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.) Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Advertisements

Napins, 2S albumins, are major allergens in oilseed rape and turnip rape Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,

Pru p 3 acts as a strong sensitizer for peanut allergy in Spain

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

Allergen immunotherapy: A practice parameter third update

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE Elizabeth A. Erwin,

Fatty acids, inflammation, and asthma

Lupine allergy: Not simply cross-reactivity with peanut or soy

Identification and immunologic characterization of an allergen, alliin lyase, from garlic (Allium sativum) Shao-Hsuan Kao, MS, Ching-Hsian Hsu, MD, PhD,

Journal of Allergy and Clinical Immunology

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Gloria García-Casado, PhD, Jesús F

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Hypersensitivity reactions due to nitrile gloves

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Is 9 more than 2 also in allergic airway inflammation?

Fatty acids, inflammation, and asthma

Allergy to eggplant (Solanum melongena)

Reassessing the role of hyaluronidase in yellow jacket venom allergy

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

Component-resolved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins∗ Ingrid Sander, PhD, Hans-Peter Rihs, PhD,

Loss of allergenic proteins during boiling explains tolerance to boiled peanut in peanut allergy Paul J. Turner, FRACP, PhD, Sam Mehr, FRACP, Rebekah.

Leticia Vila, MD, PhD, Borja Bartolome, PhD, Ana Moreno, MD

Allergy testing in predicting outcome of open food challenge to peanut

Anaphylactic reaction to (mixed) fruit juice containing dragon fruit

Respiratory sensitization of a worker to the warehouse beetle Trogoderma variabile: An index case report Jonathan A. Bernstein, MD, Marjorie S. Morgan,

Phillip Lieberman, MD, Michael Tankersley, MD

Genetically engineered hybrid proteins from Parietaria judaica pollen for allergen- specific immunotherapy Roberto González-Rioja, PhD, Ignacio Ibarrola,

Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino,

An experimental and modeling-based approach to locate IgE epitopes of plant profilin allergens Gema López-Torrejón, PhD, Araceli Díaz-Perales, PhD, Julia.

A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines David Walgraffe, PhD, Christel Mattéotti, PhD, Mohamed el Bakkoury, PhD,

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1 Christiane Hilger, PhD,

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7

Anaphylaxis caused by hidden soybean allergens in pillows

Casein-related anaphylaxis after use of an Everlast kickboxing glove

Analysis of the structure and allergenicity of recombinant pro- and mature Der p 1 and Der f 1: Major conformational IgE epitopes blocked by prodomains

Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3) Thomas Grunwald, PhD, Benjamin Bockisch, PhD,

Cockroach allergens: Coping with challenging complexity

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Anaphylaxis to apple and orange seed

Advances in the approach to the patient with food allergy

Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy Véronique Schulten, PhD,

Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen Ignacio Ibarrola, PhD, M. Carmen Arilla, PhD, Alberto.

Induced bronchospasm after handling of orange flavedo (zest)

Beer anaphylaxis Journal of Allergy and Clinical Immunology

Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions.

Recombinant allergen-based IgE testing to distinguish bee and wasp allergy Irene Mittermann, PhD, Mihaela Zidarn, MD, Mira Silar, PhD, Zora Markovic-Housley,

Cat IgA, representative of new carbohydrate cross-reactive allergens

Eggplant anaphylaxis in a patient with latex allergy

Allergy to human seminal fluid: Cross-reactivity with dog dander

Cloning, sequencing, and recombinant production of Sin a 2, an allergenic 11S globulin from yellow mustard seeds Oscar Palomares, PhD, Andrea Vereda,

Pedro Gamboa, MD, PhD, Rosa Sanchez-Monge, PhD, M

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties

Cloning, expression, and clinical significance of the major allergen from ash pollen, Fra e 1 Rodrigo Barderas, BSc, Ashok Purohit, MD, Ioanna Papanikolaou,

Macrolide antibiotics and asthma treatment

Teresa Asensio, MD, Jesus F

Wheat lipid transfer protein is a major allergen associated with baker's asthma Arantxa Palacin, PhD, Santiago Quirce, MD, PhD, Alicia Armentia, MD, PhD,

Asthma: The past, future, environment, and costs

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries Merima Bublin, MSc,

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

The effects of gastric digestion on codfish allergenicity

Natural history of cow’s milk allergy

Molecular cloning of a major Alternaria alternata allergen, rAlt a 2

Presentation transcript:

Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens Juan A. Asturias, PhD, Ignacio Ibarrola, PhD, Angel Ferrer, MD, PhD, Carmen Andreu, MD, Esperanza López-Pascual, MD, Joaquín Quiralte, MD, PhD, Fernando Florido, MD, PhD, Alberto Martínez, PhD Journal of Allergy and Clinical Immunology Volume 115, Issue 6, Pages 1210-1217 (June 2005) DOI: 10.1016/j.jaci.2005.02.012 Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 A, Coomassie-stained SDS-PAGE and immunoblots of A alternata allergens. nAlt a 1 (lanes 1), rAlt a 2 (lanes 2), and rAlt a 11 (lanes 3) were run in the presence and absence of β-mercaptoethanol (βME). Immunoblots were incubated with a human serum pool of patients with A alternata allergy. B, Dot blots for nAlt a 1, rAlt a 2, rAlt a 11, and BSA spotted onto nitrocellulose membrane. Dot blots were incubated with individual sera from patients with A alternate allergy. Journal of Allergy and Clinical Immunology 2005 115, 1210-1217DOI: (10.1016/j.jaci.2005.02.012) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 A, Coomassie-stained SDS-PAGE and immunoblot of A alternata allergens. nAlt a 1 (lanes 1), rbAlt a 1 (lanes 2), and ryAlt a 1 (lanes 3) were run in the presence and absence β-mercaptoethanol (βME). Immunoblots were incubated with a human serum pool of patients with A alternata allergy. B, EAST inhibition curves. The binding of human IgE to A alternata extract was inhibited with the following: A alternata extract, nAlt a 1, rbAlt a 1, and ryAlt a 1. Values represent means ± SD. Journal of Allergy and Clinical Immunology 2005 115, 1210-1217DOI: (10.1016/j.jaci.2005.02.012) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 SPT with A alternata extract (AAE), and 3 different concentrations of purified natural and rAlt a 1 are shown. Values are given as means of wheal surface areas. ∗Difference significant at P < .05. NS, Not significant. Journal of Allergy and Clinical Immunology 2005 115, 1210-1217DOI: (10.1016/j.jaci.2005.02.012) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions