Endothelial cell seeding of a new PTFE vascular prosthesis

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Presentation transcript:

Endothelial cell seeding of a new PTFE vascular prosthesis Richard F. Kempczinski, M.D., John E. Rosenman, M.D., William H. Pearce, M.D., L.Richard Roedersheimer, M.D., Yacov Berlatzky, M.D., Georges Ramalanjaona, M.D. Journal of Vascular Surgery Volume 2, Issue 3, Pages 424-429 (May 1985) DOI: 10.1016/0741-5214(85)90096-5 Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 Luminal surface of control (A) and endothelial cell—seeded graft (B) in same animal 6 weeks after implantation. Note thick, exuberant thrombus and absence of endothelium, beyond pannus ingrowth, on control graft, which nevertheless remained patent. By contrast note thin, glistening lining and absence of thrombus on seeded graft. Journal of Vascular Surgery 1985 2, 424-429DOI: (10.1016/0741-5214(85)90096-5) Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Three-dimensional view of seeded graft seen with scanning electron microscope. Note intact endothelial lining on luminal surface (L) and substantial subendothelial layer (SE) attached to body of graft (G). (Original magnification × 190.) Journal of Vascular Surgery 1985 2, 424-429DOI: (10.1016/0741-5214(85)90096-5) Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 Transmission electron micrograph of luminal surface of seeded graft shows confluent endothelial cells with tight junctions and surface microvilli. Note also subendothelial smooth muscle cells and collagen fibrils. (Original magnification × 3900.) Journal of Vascular Surgery 1985 2, 424-429DOI: (10.1016/0741-5214(85)90096-5) Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions