Dose-response curves and images for confirmed screening hits.

Slides:

Advertisements

Similar presentations

Loratadine Loratadine is a histamine H1 receptor antagonist, used to treat allergies. Also acts as a selective inhibitor of B(0)AT2 with IC50 of 4 μM.

Advertisements

Mitochondria localize to both the front and the rear of neutrophils.

TMZ and Ara-C treatment of slice co-cultures.

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Inhibition of PI3K or SERCA phenocopies polycystin loss of function.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA treatment of Renca cells. Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA.

An antagonistic relationship between myosin II and dynein controls bulk growth cone advance. An antagonistic relationship between myosin II and dynein.

Localisation of epithelial markers in mMEC cultures.

Representative histological images of aorta from WT, ApoEm/m and ApoE−/− pigs. Representative histological images of aorta from WT, ApoEm/m and ApoE−/−

Fig. 4. A primary screen based on scrape closure

Fig. 4. BMP and nodal induce invasion of metastatic and radial growth phase melanoma cells in human epidermal skin reconstructs. BMP and nodal induce invasion.

X-ray radiographic images showing structural alterations of the joints that are most commonly affected by OA. (A) Normal and (A′) severely affected joint.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Scanning EM shows cilia abnormalities in the neural tube.

Hipk induces cell spreading.

Challenge with oxazolone results in epithelial hyperplasia and influx of inflammatory cells into the epidermis and dermis and keratosis as observed in.

Characterization of Col1a1FRT-STOP-FRT-Cre-ER-T2 mice.

Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice. Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice.

Latency to onset of Stage III behavioral seizure activity.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W embryos at E16.5. Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W.

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Hipk induces overgrowths in Drosophila imaginal discs.

Treatment of HCMT-CMs with bisoprolol.

Gibberellin responses.

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. Overexpression of individual.

The effect of TDP-43A315T expression on dendrite spine development.

The mutants exhibit congenital hypothyroidism.

An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic.

Chemical chaperone 4-PBA rescues the hi559 GI phenotype.

The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.

Characterization of zebrafish muscle wasting in krasG12V-induced HCC zebrafish. 4-month-old male adult krasG12V and WT zebrafish were treated with dox.

Cellular reaction to intraventricular DMSO injection.

Rgs16::GFP expression in pancreatic neoplasia.

Effect of the plastid translation inhibitor Spec on LR development

Tissue ATP levels measured in the liver, muscle, kidney and pancreas of CDs/y and CDr/y on RD or DD. *P

Transbilayer distribution of cholesterol in the plasma membrane is defective in staurosporine-treated cells. Transbilayer distribution of cholesterol in.

Induction of liver tumors in TO(Myc) fish.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

Tumor location determines response to intravenous FOLFIRINOX chemotherapy. Tumor location determines response to intravenous FOLFIRINOX chemotherapy. (A)

Nerve conduction slowing and DRG sensory neuron and nuclear atrophy in 16-week-old diabetic mice. Nerve conduction slowing and DRG sensory neuron and nuclear.

N-cadherin deficient cells do not adhere or migrate.

Food-additive exposure increases permeability in a human gut cell co-culture model. Food-additive exposure increases permeability in a human gut cell co-culture.

Effect of γ-secretase inhibition on the vulnerability of primary neurons to OGD. (A) Experimental design. Effect of γ-secretase inhibition on the vulnerability.

Deletion of Sox2 in adult mice inhibits incisor regeneration.

Optimized STZ treatment leads to specific cytotoxicity of insulin-producing cells. Optimized STZ treatment leads to specific cytotoxicity of insulin-producing.

HEK-cFXN cells show a correlation among ROS levels, LIP and frataxin expression. HEK-cFXN cells show a correlation among ROS levels, LIP and frataxin expression.

Ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis. ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis.

Regulation of lipid droplet formation by PI3-kinase activity.

ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. (A,B)

VEGF stimulation together with inhibition of mTORC1 induces endothelial cell elongation independently of Foxo1. VEGF stimulation together with inhibition.

Peroxisome speeds were slower in patient and control cells.

Fig. 2. Acetylation stiffens primary cilia.

Proliferation on soft matrices is decoupled from myosin light chain phosphorylation, cell tractions, and focal adhesions. Proliferation on soft matrices.

PtdIns(4,5)P2 dependence of TbEpsinR and TbCALM membrane targeting.

Effect of siRNA transfection on colony formation by Renca cells.

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Maytansinol disrupts the mitotic spindle and prevents mitotic exit in Drosophila. Maytansinol disrupts the mitotic spindle and prevents mitotic exit inDrosophila.

Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

A normal/intermediate activity of calmodulin-calcineurin pathway is required to cope with SDS stress in vps13Δ. A normal/intermediate activity of calmodulin-calcineurin.

Functional and molecular regeneration in mdx muscle with matrix treatment. Functional and molecular regeneration in mdx muscle with matrix treatment. (A,B)

Upon challenge with oxazolone, human leukocytes (mainly consisting of T cells) infiltrate the dermis and epidermis. Upon challenge with oxazolone, human.

Presentation transcript:

Dose-response curves and images for confirmed screening hits. Dose-response curves and images for confirmed screening hits. (A-F) Treatment with the NMDA receptor antagonist ifenprodil (B, 5 µM), the myosin inhibitor blebbistatin (C, 5 µM), the smooth muscle relaxant alverine citrate (D, 5 µM), or the long chain fatty acid pentadecanoic acid (E, 5 µM) all increased measures of neurite growth compared to DMSO treated controls (A), whereas the plant-derived natural diterpene triptolide (F, 1 µM) reduces neurite outgrowth without affecting cell number. Scale bar: 50 µm. Data are mean±s.d., n=6 wells (mean, 244 cells/well). Sean P. Sherman, and Anne G. Bang Dis. Model. Mech. 2018;11:dmm031906 © 2018. Published by The Company of Biologists Ltd