Cathepsin D in wild-type and Nuc1 RPE

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Invest. Ophthalmol. Vis. Sci ;44(9): doi: /iovs Figure Legend:

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Cathepsin D in wild-type and Nuc1 RPE Cathepsin D in wild-type and Nuc1 RPE. Western blotting with cathepsin D antibody. Cathepsin D in wild-type and Nuc1 RPE. Western blotting with cathepsin D antibody. RPE samples were as follows: Lane 1, 2-month-old wild type; lane 2, 2-month-old Nuc1; lane 3, 8-month-old wild type; lane 4, 8-month-old Nuc1. The immature (inactive) pro-enzyme form of cathepsin is present in all samples. The mature (active) form of the enzyme is present in the wild-type samples, but is barely detectable in the Nuc1 samples. Actin is shown as a loading control. J. Samuel Zigler, Jr et al. J Cell Sci 2011;124:523-531 © 2011.