The risk allele of rs56048322 affects TCR-mediated intracellular calcium flux in CD4+ T cells from subjects with T1D or RA. Previously frozen PBMCs from.

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The risk allele of rs56048322 affects TCR-mediated intracellular calcium flux in CD4+ T cells from subjects with T1D or RA. Previously frozen PBMCs from a healthy subject and from subjects with T1D or RA were loaded with cell-permeant indo-1, AM dye and stained with antibodies against T-cell surface markers. The risk allele of rs56048322 affects TCR-mediated intracellular calcium flux in CD4+ T cells from subjects with T1D or RA. Previously frozen PBMCs from a healthy subject and from subjects with T1D or RA were loaded with cell-permeant indo-1, AM dye and stained with antibodies against T-cell surface markers. Shown are kinetics profiles depicting the mean indo-1 ratio (violet/blue) as a function of time in gated total CD4+ T cells before and after stimulation with 10 μg/mL anti-CD3 antibody. Percent maximal calcium flux is expressed as [(peak anti-CD3 flux − mean baseline)/(peak ionomycin flux − mean baseline)] × 100. All the subjects are homozygous for the major nonrisk allele of rs2476601 in PTPN22. Yan Ge et al. Diabetes 2016;65:794-802 ©2016 by American Diabetes Association