The autophagy pathway is not required for STx2B trafficking. The autophagy pathway is not required for STx2B trafficking. (A, B) Genomic DNA sequences. STX17, syntaxin17. (C, D, K, N) RT–PCR. (E, H, L, O) STx2B transport imaged at 0 or 60 min. Scale bars, 25 μm. (F, I) Pearson’s coefficient for colocalization between STx2B and the Golgi apparatus at 60 min from (E) and (H). N = 15 cells per condition. There were no differences between groups using t test. (G, J, M, P) STx2B levels from (E, H, L, O). Levels at 0 min in WT cells (G, J) or cells transfected with control siRNA (M, P) normalized to 100. N > 15 cells per condition. There were no differences between WT or control siRNA-transfected cells at 0 min and other groups using one-way ANOVA and Dunnett’s post hoc test. Andrey S Selyunin et al. LSA 2019;2:e201900439 © 2019 Selyunin et al.