Spiking venom with rVes v 5 improves sensitivity of IgE detection in patients with allergy to Vespula venom Byrthe Vos, Julian Köhler, Sabine Müller,

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Presentation transcript:

Spiking venom with rVes v 5 improves sensitivity of IgE detection in patients with allergy to Vespula venom Byrthe Vos, Julian Köhler, Sabine Müller, MD, Eva Stretz, Franziska Ruëff, MD, Thilo Jakob, MD Journal of Allergy and Clinical Immunology Volume 131, Issue 4, Pages 1225-1227.e1 (April 2013) DOI: 10.1016/j.jaci.2012.07.041 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 IgE antibody levels in patients with allergy to YJ sting (n = 308). A, IgE reactivity to YJV extract (ImmunoCAP i3), rVes v 1 (i211), and rVes v 5 (i209) in the entire population. B, IgE reactivity to rVes v 1 (i211) and rVes v 5 (i209) in the YJV extract (i3)–negative population. Journal of Allergy and Clinical Immunology 2013 131, 1225-1227.e1DOI: (10.1016/j.jaci.2012.07.041) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Comparison of IgE reactivity to conventional YJV extract, recombinant YJ allergens Ves v 1 and Ves v 5, and to Ves v 5-spiked YJV extract in patients with YJV allergy. A, Comparison of IgE levels with YJV ImmunoCAP (i3) and rVes v 1 (i211), all patients (n = 308). B, Comparison of IgE levels with YJV ImmunoCAP (i3) and rVes v 5 (i209), all patients (n = 308). C, Comparison of IgE levels with YJV ImmunoCAP (i3) and rVes v 5-spiked YJV extract in rVes v 5–positive patients (n = 277). D, Comparison of IgE levels with YJV ImmunoCAP (i3) and rVes v 5-spiked YJV extract in rVes v 5–negative patients (n = 31). Hatched horizontal and vertical lines indicate the cutoff of 0.35 kUA/L, and the hatched diagonal line represents a 1:1 ratio. Journal of Allergy and Clinical Immunology 2013 131, 1225-1227.e1DOI: (10.1016/j.jaci.2012.07.041) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Comparison of IgE levels with YJV ImmunoCAP (i3) and rVes v 5-spiked YJV extract in patients with allergy to honeybee venom. A, Entire population (n = 51). B, rVes v 5–negative patients (n = 38). C, rVes v 5–positive patients (n = 13). Hatched horizontal and vertical lines indicate the cutoff of 0.35 kUA/L, and the hatched diagonal line represents a 1:1 ratio. Journal of Allergy and Clinical Immunology 2013 131, 1225-1227.e1DOI: (10.1016/j.jaci.2012.07.041) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions