FeTTPS blocks tyrosine nitration of TrkA receptor and restores TrkA-Y490 phosphorylation in RGCs. A: Peroxynitrite (PN) increased TrkA tyrosine nitration.

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FeTTPS blocks tyrosine nitration of TrkA receptor and restores TrkA-Y490 phosphorylation in RGCs. A: Peroxynitrite (PN) increased TrkA tyrosine nitration (more than twofold) as determined by immunoprecipitation (I.P.) of the TrkA and immunoblotting (I.B.) with the nitrotyrosine (NY) antibody. FeTTPS blocks tyrosine nitration of TrkA receptor and restores TrkA-Y490 phosphorylation in RGCs. A: Peroxynitrite (PN) increased TrkA tyrosine nitration (more than twofold) as determined by immunoprecipitation (I.P.) of the TrkA and immunoblotting (I.B.) with the nitrotyrosine (NY) antibody. Equal loading was demonstrated by immunoblotting with TrkA antibody. Treatment of RGCs with NGF (50 ng/ml) maintained tyrosine nitration of TrkA. B: Western blot analysis showing that peroxynitrite (100 μmol/l) diminished NGF-induced TrkA phosphorylation at Y490 that was restored by treatment with FeTPPS (2.5 μmol/l). C: Western blot analysis showing that peroxynitrite (100 μmol/l) decreased NGF-induced Akt activation (P-Akt), a downstream target of TrkA survival signal. Treatment with FeTPPS restored Akt activity and NGF prosurvival signal. Data shown are means ± SD of four per group. *P < 0.05 compared with controls; #P < 0.05 compared with NGF-treated samples. Tayyeba K. Ali et al. Diabetes 2008;57:889-898 ©2008 by American Diabetes Association