Evaluation of different techniques for washing cats: Quantitation of allergen removed from the cat and the effect on airborne Fel d 1 David B. Avner,

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Evaluation of different techniques for washing cats: Quantitation of allergen removed from the cat and the effect on airborne Fel d 1 David B. Avner, BA, Matthew S. Perzanowski, BA, Thomas A.E. Platts-Mills, MD, PhD, Judith A. Woodfolk, MB, ChB Journal of Allergy and Clinical Immunology Volume 100, Issue 3, Pages 307-312 (September 1997) DOI: 10.1016/S0091-6749(97)70242-2 Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Time course of Fel d 1 extraction from 0.05 gm aliquots of cat hair with borate-buffered saline, pH 8 (filled squares); water (filled triangles); shampoo, pH 7 (open circles); and 1 mmol/L HCl, pH 3 (open triangles). Values represent the mean of 10 samples. Journal of Allergy and Clinical Immunology 1997 100, 307-312DOI: (10.1016/S0091-6749(97)70242-2) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Airborne Fel d 1 (nanograms per cubic meter) derived from cats in a 30 m3 room before (filled triangles) and 3 hours after (open circles) washing by immersion in 30 L of water. Values represent the mean for three cats. *Total allergen removed by washing (in milligrams). Bars represent 1 standard deviation from the mean. Airborne levels were significantly reduced 3 hours after washing (n < 0.001). Journal of Allergy and Clinical Immunology 1997 100, 307-312DOI: (10.1016/S0091-6749(97)70242-2) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Airborne Fel d 1 (nanograms per cubic meter) derived from two cats studied separately in a 30 m3 room before (filled triangles) and 3 hours after (filled circles) washing, followed by rinsing. In one case a progressive reduction in airborne allergen levels was detected (A), and in the other the decrease after washing was not maintained 1 week later (B). *Total allergen removed by washing (in milligrams). Journal of Allergy and Clinical Immunology 1997 100, 307-312DOI: (10.1016/S0091-6749(97)70242-2) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Airborne Fel d 1 (nanograms per cubic meter) derived from two cats studied separately in a 30 m3 room before (filled triangles) and 3 hours after (filled circles) washing, followed by rinsing. In one case a progressive reduction in airborne allergen levels was detected (A), and in the other the decrease after washing was not maintained 1 week later (B). *Total allergen removed by washing (in milligrams). Journal of Allergy and Clinical Immunology 1997 100, 307-312DOI: (10.1016/S0091-6749(97)70242-2) Copyright © 1997 Mosby, Inc. Terms and Conditions