Induction of MalE expression by Cpd-1 in RAM2808.

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Induction of MalE expression by Cpd-1 in RAM2808. Induction of MalE expression by Cpd-1 in RAM2808. RAM2808 cells were grown with (top) or without (bottom) 0.4% maltose and induced with 0.2% arabinose (Ara, expression of LamB). The bacterial suspensions were incubated with Cpd-1. Samples were recovered at various time points and a cellular fractionation protocol was performed. Controls are the same bacterial suspensions incubated without Cpd-1 recovered at 30 min. Presence of MalE in the periplasmic fraction was monitored over time by Western blot analysis. Estelle Dumont et al. LSA 2019;2:e201800242 © 2018 Dumont et al.