Reconstituted Xenopus Lnp forms stacked membrane discs.

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Reconstituted Xenopus Lnp forms stacked membrane discs. Reconstituted Xenopus Lnp forms stacked membrane discs. (A) Domain organization of Lnp. CC1, CC2, coiled-coil domains 1 and 2; TM1 and TM2, transmembrane segments 1 and 2; P, phosphorylation domain; Zn2+, Zn2+-finger domain. Domains are colored according to the degree of sequence conservation. (B) Purified His10-tagged Xenopus Lnp was reconstituted with phospholipids at a protein-to-lipid molar ratio of 1:5 and visualized by negative-stain EM. The lower panel shows a magnified view. Scale bar, 20 nm. (C) As in (B), but with different protein-to-lipid ratios. Scale bar, 20 nm. Songyu Wang et al. LSA 2018;1:e201700014 © 2018 Wang et al