Supplementary Table Primer Sequence Reference Region 1 BS-CTCF-725-F

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Supplementary Table Primer Sequence Reference Region 1 BS-CTCF-725-F BS-CTCF-725-F GTTTTTGGGATATAGTTGAGTT BS-CTCF-725-R ATACTAAAAAACACAATACTTTCA Region 2 Bs-CTCF-bishortF TTTTTTTTGGGAGTAGGTAGAA AACTACAACTCCCAACAACC Region 3 Bs-CTCF-CoreR CTAAATACCTATATCAATACTAAA Bs-CTCF-CoreF GTTTTTATTAAGTTTTAGGGTTTT Region 4 Kang et al. 2001. Lab Invest. 81: 573-9 BsP53promF TTATAGTTTTGGTTTGTAGAAT BsP53promR nested BsnesP53promF TTTTTATTTTAAAATGTTAGTA ATCAAATTCAATCAAAAACTTA Region 5 BsdownP53P1F GAGTAGGTAGTTGTTGGGTTT BsdownP53P1R TATACATCTTTTATTTCAAAAATTCC Region 6 BsdownP53P2F GGGAGTTTAAGTTGTTAAGGTTT BsdownP53P2R CAAATACCCCTACCTTTACAAAA Region 7 Bs-islaCpGDownF TTTTGGGTTAGGTTATTTTAGTT Bs-islaCpGDownR AACCAAAACTCATCTTCCTATAC Controls ENDOGENOUS DNA METHYLATION ANALYSIS Alu (Accession U14574) Brown, et al. 2007. Epigenetics 2:1, 54-65. Bs- Alu-sense TTTTTATTAAAAATATAAAAATTAGT Bs-Alu-anti-sense CCCAAACTAAAATACAATAA FZD9 gene BS-FZD9 FWR GTTTTTAAAGTTTAGTGGGAGG BS-FZD9-REV ATAACCCAACAAATTAAACATAC IN VITRO DNA METHYLATION ANALYSIS P53 IVD We use the same primers as the p53 promoter (Region 4), using DNA from normal Lymphocytes in vitro methylated with SssI for 8 hours Supplementary Table