Mass cytometry analysis of wt, mdx, and ctx FAPs.

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Mass cytometry analysis of wt, mdx, and ctx FAPs. Mass cytometry analysis of wt, mdx, and ctx FAPs. FAPs were purified using MACS microbead technology from hind limb muscles of 6-wk-old wt, mdx, and ctx (3 dpostinjury) mice. The different cell samples were bar coded by labeling with different proportions of three palladium isotopes. The bar coded cell samples were pooled and incubated with 12 metal-labeled antibodies (SCA1, CD34, CD146, CD140b, CD31, CXCR4, CAS3, Vim, pSTAT1, TNFa, pCREB and pSTAT3) and analyzed by mass cytometry in a Cytof2 instrument. (A) Schematic representation of the experimental design. (B) Intensity distribution of the signals of four different antigens (SCA1, CD34, CD146 and CD140b). (C) Contour maps of the scatter plots showing the correlations between SCA1 and CD34 expression in the three biological repeats for the three conditions. Colors represent cell densities:red = high, blue = low. The two different identified subpopulations are outlined by green (SCA1HCD34H) or yellow (SCA1LCD34L) ovals. (D) Contour representations of the viSNE maps in the nine different cell preparations. Regions of interest identifying cell subpopulations that characterize the different biological conditions are circled (A to E in the top left panel). Milica Marinkovic et al. LSA 2019;2:e201900437 © 2019 Marinkovic et al.