N-cadherin deficient cells do not adhere or migrate.

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N-cadherin deficient cells do not adhere or migrate. N-cadherin deficient cells do not adhere or migrate. (A) Western blot and immunofluorescence staining of N-cadherin for HGF-treated wild-type (WT) and two selected N-cadherin knockdown cells. KD1 clone with >99%, and KD2 clone with ∼90% knockdown of N-cadherin. (B) Representative images and quantification of cell aggregation in a suspension using hanging drop analysis. CD, cytochalasin-D-treated cells. (C) 3D cell migration analysis of wild-type (WT) and N-cadherin knockdown (KD1 and KD2) cells. (D) Average speeds of wild-type (WT) cells as single cells (S) or cells in cluster (C), and knockdown cells (KD1, n = 32 and KD2, n = 24). (E) Endpoint displacements of wild-type and knockdown cells in a 3D collagen matrix. Scale bars: 10 µm (A), 20 µm (B,C). Wenting Shih, and Soichiro Yamada J Cell Sci 2012;125:3661-3670 © 2012. Published by The Company of Biologists Ltd